Sample plate systems and methods

• US 9,523,701 B2
• Filed: 07/21/2011
• Issued: 12/20/2016
• Est. Priority Date: 07/29/2009
• Status: Active Grant

• Current Assignee: Dynex Technologies, Inc.
• Original Assignee: Dynex Technologies, Inc.
• Inventors: Bunce, Adrian, Fusellier, Andrew
• Primary Examiner(s): LAM, ANN Y
• Application Number:
  US13/187,791
• Publication Number:
  US 20120183977A1
• Time in Patent Office: 1,979 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00459 Beads
• CPC Class Codes: B01J 2219/00468 by manipulation of individual beads
• CPC Class Codes: B01J 2219/005 Beads
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01L 2200/0668 Trapping microscopic beads
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 2035/00574 Means for distributing beads
• CPC Class Codes: G01N 2035/1086 Cylindrical, e.g. variable angle
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G01N 35/1081 characterised by the means for relatively moving the transfer device and the containers in an horizontal plane G01N35/1011 takes precedence

Abstract

A sample plate comprising a sample well is disclosed. The sample well can comprise one or more bead retaining chambers. A method of using the sample plate and a kit comprising the sample plate is also disclosed.

Claims

1. A sample plate comprising one or more sample wells, wherein one or more of said sample wells comprise:

(a) a base portion; and

(b) two or more recesses provided in said base portion;

wherein each of said two or more recesses has a dimension and contains a bead deposited in said well to be substantially retained or secured within said recess, and said bead forms a substantially fluid-tight circumferential seal with a wall of said base portion which defines said recess.

2. The sample plate of claim 1, wherein each of said two or more recesses comprise a blind recess or an open through hole.

3. The sample plate of claim 1, wherein said recess is substantially cylindrical.

4. The sample plate of claim 1, wherein an opening to said recess is circular.

5. The sample plate of claim 1, wherein said recess is conical and has a first diameter which is greater than a diameter of a bead deposited in said recess and a second diameter which is less than a diameter of said bead deposited in said recess.

6. The sample plate of claim 5, wherein said through recess has a taper selected from the group consisting of:

(i)<

0.5.degree.;

(ii) 0.5.degree.;

(iii) 0.5-1.degree.;

(iv) 1-2.degree.;

(v) 2-4.degree.;

(vi) 4-6.degree.;

(vii) 6-8.degree.;

(viii) 8-10.degree.; and

(ix) >

10.degree.

7. The sample plate of claim 1, wherein the diameter or depth of said recess is selected from the group consisting of:

(i)<

0.5 mm;

(ii) 0.5-1.0 mm;

(iii) 1.0-1.5 mm;

(iv) 1.5-2.0 mm;

(v) 2.0-2.5 mm;

(vi) 2.5-3.0 mm;

(vii) 3.0-3.5 mm;

(viii) 3.5-4.0 mm;

(ix) 4.0-4.5 mm;

(x) 4.5-5.0 mm;

(xi)<

5.0 mm; and

(xii) >

5.0 mm.

8. The sample plate of claim 1, wherein in at least one sample well said base portion is segmented into a plurality of segments which are arranged at different heights relative to each other.

9. The sample plate of claim 1, wherein in at least one sample well, said sample well further comprises one or more baffles or dividers which separates or divides said base portion into at least a first region and a second region.

10. The sample plate of claim 9, wherein said one or more baffles or dividers attenuate or eliminate light reflected off one or more reagent beads located in said first region from impinging upon one or more reagent beads located in said second region.

11. The sample plate of claim 1, wherein said two or more recesses comprise a countersunk or enlarged portion for facilitating the insertion of a bead into one or more of said through holes or recesses.

12. The sample plate of claim 1, wherein said one or more sample wells comprises between 2 and 22 recesses.

13. The sample plate of claim 12, wherein said two or more recesses are arranged circumferentially around a central portion of said sample well.

14. The sample plate of claim 13, wherein said central portion comprises a central recess.

15. The sample plate of claim 14, wherein said central portion does not comprise a recess.

16. The sample plate of claim 13, wherein said plurality of recesses is arranged in a substantially symmetrical or regular manner.

17. The sample plate of claim 13, wherein said two or more recesses is arranged in a substantially asymmetrical or irregular manner.

18. The sample plate of claim 13, wherein said two or more recesses is arranged in a substantially linear manner.

19. The sample plate of claim 13, wherein said two or more recesses is arranged in a substantially curved manner.

20. The sample plate of claim 1, wherein said sample plate comprises sample wells arranged in an A×

B format, wherein A and B are perpendicular axes, and the number of wells along said A axis can be greater than, less than, or equal to the number of wells along said B axis.

21. The sample plate of claim 20, wherein said number of wells along said A axis or B axis is at least 2.

22. The sample plate of claim 20, wherein said number of wells along said A axis or B axis is between 2 and 15.

23. The sample plate of claim 1, wherein one of said sample wells is connected to another sample well by a frangible region.

24. The sample plate of claim 1, wherein said sample plate comprises a base comprising a docking portion for securing said sample plate to a corresponding docking portion of a plate frame holder.

25. The sample plate of claim 1, further comprising a bead.

26. The sample plate of claim 25, wherein said bead is attached to a probe.

27. The sample plate of claim 26, wherein said probe is a nucleic acid, antibody, antibody fragment, protein, peptide, aptamer, or chemical compound.

28. A bead dispensing system comprising:

(a) a plurality of beads;

(b) a bead dispenser for dispensing at least some of said plurality of beads;

(c) a sample plate comprising a sample well, wherein said sample well comprises a base portion, wherein said base portion comprises one or more recesses, wherein each of said one or more recesses has a dimension for a bead deposited in said well to be substantially retained or secured within said recess, and said bead forms a substantially fluid-tight circumferential seal with a wall of said base portion which defines said recess; and

(d) a control system configured to control dispensing of said bead from said bead dispenser into said sample plate.

29. The bead dispensing system of claim 28, wherein said one or more recesses comprise a blind recess or an open through hole.

30. The bead dispensing system of claim 28, wherein said bead dispenser comprises:

(i) a syringe body comprising an annular chamber surrounding a longitudinal bore, wherein said annular chamber is configured to channel a reagent bead within said annular chamber towards a chamber provided in said bore;

(ii) a plunger provided within said longitudinal bore; and

(iii) a barrel or nozzle;

wherein said plunger is configured to dispense a bead of said plurality of beads from said chamber into said barrel or nozzle.

31. The bead dispensing system of claim 28, wherein said bead dispenser is configured to dispense the plurality of beads automatically.

32. A method of dispensing beads comprising:

(a) providing a bead dispenser comprising a bead;

(b) providing a sample plate comprising a sample well, wherein said sample well comprises a base portion;

wherein said base portion comprises two or more recesses, wherein each of said two or more recesses has a dimension for a bead deposited in said well to be substantially retained or secured within said recess, and said bead forms a substantially fluid-tight circumferential seal with a wall of said base portion which defines said recess; and

(c) controlling the dispensing of said bead from said bead dispenser into said sample plate.

33. The method of claim 32, wherein said two or more recesses comprise a blind recess or an open through hole.

34. The method of claim 32, wherein said dispensing is performed automatically.

35. A kit for detecting an analyte comprising:

(a) a plurality of beads; and

(b) sample plate comprising a sample well, wherein said sample well comprises a base portion;

wherein said base portion comprises one or more recesses, wherein each of said one or more recesses has a dimension for a bead deposited in said well to be substantially retained or secured within said recess, and said bead forms a substantially fluid-tight circumferential seal with a wall of said base portion which defines said recess.

36. The kit of claim 35, wherein said one or more recesses comprise a blind recess or an open through hole.

37. The kit of claim 35, wherein said plurality of beads comprise one or more probes.

38. The kit of claim 37, wherein said probe is a nucleic acid, antibody, antibody fragment, protein, peptide, aptamer, or chemical compound.

39. A method of detecting an analyte comprising:

(a) adding a sample to a sample plate comprising a sample well, wherein said sample well comprises a base portion;

wherein said base portion comprises two or more recesses, wherein each of said two or more recesses has a dimension for a bead deposited in said well to be substantially retained or secured within said recess, wherein at least one of said one or more recesses contains a bead and said bead forms a substantially fluid-tight circumferential seal with a wall of said base portion which defines said recess, and wherein said bead comprises a probe; and

(b) detecting binding of an analyte in said sample with said probe.

40. The method of claim 39, wherein each of said two or more recesses comprises a blind recess and an open through hole.

41. The method of claim 39, wherein said sample plate comprises a plurality of probes and a plurality of analytes are detected.

42. The method of claim 39, wherein a plurality of samples is added to said sample plate.

43. A method of manufacturing a sample plate comprising:

(a) providing a sample plate comprising one or more sample wells each having a base portion; and

(b) forming two or more recesses in said one or more base portions, wherein each of said two or more recesses has a dimension and contains a bead deposited in said well, in use, to be substantially retained or secured within said recess, and said bead, in use, forms a substantially fluid-tight circumferential seal with a wall of said base portion which defines said recess.

44. The method of claim 43, wherein each of said two or more recesses comprises a blind recess or an open through hole.