Processes for detecting or quantifying more than one nucleic acid in a library
First Claim
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1. A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of:
- a) providing;
(i) an array of fixed or immobilized nucleic acids identical or complementary in part or whole to sequences of said nucleic acids of interest;
(ii) a library of nucleic acid analytes which may contain the nucleic acids of interest sought to be detected or quantified; and
(iii) a first set of primers, a second set of primers and a third set of primers, wherein said first set of primers are fixed or immobilized to a solid support, and wherein said second set of primers comprises at least one production center; and
(iv) polymerizing means for synthesizing nucleic acid copies of said nucleic acid analytes using said sets of primers;
b) contacting said library of nucleic acid analytes with said first set of primers to form more than one first bound entity;
c) extending said bound first set of primers by means of template sequences provided by said nucleic acid analytes to form first copies of said analytes;
d) contacting said extended first copies with said second set of primers to form more than one second bound entity;
e) extending said bound second set of primers by means of template sequences provided by said extended first copies to form an extended second set of primers;
f) separating said extended second set of primers obtained in step e);
g) contacting said extended second set of primers with said third set of primers to form more than one third bound entity;
h) extending said third bound entity by means of template sequences provided by said extended second set of primers to form more than one complex comprising said extended third bound entity and said extended set of primers;
i) synthesizing from a production center in said second set of primers in said complexes one or more nucleic acid copies under isothermal or isostatic conditions;
j) hybridizing said nucleic acid copies formed in step i) to said array of nucleic acids provided in step a) (i); and
k) detecting or quantifying any of said hybridized copies obtained in step j).
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Abstract
This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
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Citations
39 Claims
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1. A process for detecting or quantifying more than one nucleic acid of interest in a library comprising the steps of:
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a) providing; (i) an array of fixed or immobilized nucleic acids identical or complementary in part or whole to sequences of said nucleic acids of interest; (ii) a library of nucleic acid analytes which may contain the nucleic acids of interest sought to be detected or quantified; and (iii) a first set of primers, a second set of primers and a third set of primers, wherein said first set of primers are fixed or immobilized to a solid support, and wherein said second set of primers comprises at least one production center; and (iv) polymerizing means for synthesizing nucleic acid copies of said nucleic acid analytes using said sets of primers; b) contacting said library of nucleic acid analytes with said first set of primers to form more than one first bound entity; c) extending said bound first set of primers by means of template sequences provided by said nucleic acid analytes to form first copies of said analytes; d) contacting said extended first copies with said second set of primers to form more than one second bound entity; e) extending said bound second set of primers by means of template sequences provided by said extended first copies to form an extended second set of primers; f) separating said extended second set of primers obtained in step e); g) contacting said extended second set of primers with said third set of primers to form more than one third bound entity; h) extending said third bound entity by means of template sequences provided by said extended second set of primers to form more than one complex comprising said extended third bound entity and said extended set of primers; i) synthesizing from a production center in said second set of primers in said complexes one or more nucleic acid copies under isothermal or isostatic conditions; j) hybridizing said nucleic acid copies formed in step i) to said array of nucleic acids provided in step a) (i); and k) detecting or quantifying any of said hybridized copies obtained in step j). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39)
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Specification