Reagent patterning in capillarity-based analyzers and associated systems and methods

• US 9,528,987 B2
• Filed: 06/25/2012
• Issued: 12/27/2016
• Est. Priority Date: 06/23/2011
• Status: Expired due to Fees

• Current Assignee: University of Washington
• Original Assignee: University of Washington
• Inventors: Yager, Paul, Lutz, Barry R., Fu, Elain S., Fridley, Gina, Le, Huy Quang, Kauffman, Peter C.
• Primary Examiner(s): PRIEST, AARON A
• Application Number:
  US14/129,078
• Publication Number:
  US 20140227707A1
• Time in Patent Office: 1,646 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2300/069 Absorbents; Gels to retain a fluid
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0861 Configuration of multiple channels and/or chambers in a single devices
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 2400/086 using baffles or other fixed flow obstructions
• CPC Class Codes: B01L 2400/088 by specific surface properties
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: C12Q 1/6844 Nucleic acid amplification reactions
• CPC Class Codes: G01N 33/54388 based on lateral flow
• CPC Class Codes: G01N 33/56905 Protozoa

Abstract

The present technology describes various embodiments of devices for processing, analyzing, detecting, measuring, and separating fluids. The devices can be used to perform these processes on a microfluidic scale, and with control over fluid and reagent transport. In one embodiment, for example, a device for performing chemical processes can include a porous wick comprising a pathway defined by an input end, an output end, and a length between the input end and the output end. The pathway is configured to wick fluid from the input end to the output end by capillary action. The device can further include a reagent placed on the pathway. The reagent can be placed in a pattern configured to control a spatial or temporal distribution of the reagent along the pathway upon wetting of the pathway.

Claims

1. A device for performing chemical processes, the device comprising:

a porous wick comprising a pathway defined by an input end, an output end, a length between the input end and the output end, and a pathway width, wherein the pathway is configured to wick fluid from the input end to the output end by capillary action;

a first reagent placed on the pathway, wherein the reagent is placed in a pattern configured to control a spatial or temporal distribution of the first reagent along the pathway upon flow of fluid along the pathway; and

a second reagent placed in a ring around the first reagent on the pathway.

2. The device of claim 1 wherein the first reagent is placed on a first portion of the pathway, and the second reagent is placed on a second portion of the pathway spaced apart from the first portion.

3. The device of claim 1 wherein the pathway is a first pathway, and the device further comprises a second pathway and a third reagent placed on the second pathway.

4. The device of claim 1 wherein the first reagent comprises at least one of a gold enhancement reagent, a labeled antibody, an enzymatic substrate, a silver enhancement reagent, a nucleic acid amplification reagent, trehalose, or sucrose.

5. The device of claim 1, further comprising at least one of a volume-metering element or a flow-metering element, wherein the volume-metering element or flow metering element is integral to the porous wick and is positioned to modify a rate or volume of fluid flow along the porous wick.

6. The device of claim 5 wherein the flow-metering element comprises a soluble barrier along the porous wick.

7. The device of claim 5 wherein the flow-metering element comprises a switchable barrier along the porous wick.

8. The device of claim 5 wherein the volume-metering element or the flow-metering element comprises a geometric characteristic of the pathway.

9. The device of claim 1 wherein the pathway comprises a first pathway, and wherein the porous wick further comprises a second pathway in fluid communication with the first pathway, and wherein the second pathway has a second length defined by a second input end and a second output end, and wherein the second pathway has a second length less than the length of the first pathway.

10. The device of claim 1 wherein the first reagent is placed in an array pattern configured to generate a near-uniform reagent distribution across the width of the pathway upon wetting of the pathway.

11. The device of claim 1 wherein the pathway comprises a first pathway, and wherein the device further comprises:

a second pathway defined by a second input end, a second output end, and a second length between the second input end and the second output end; and

an intersection where the first pathway and the second pathway meet and form a common pathway, wherein the common pathway comprises a receiving region, an extraction region, an amplification region, and a detection region.

12. The device of claim 1 wherein the second reagent is configured to delay the temporal distribution of the first reagent along the pathway and/or fan out the spatial distribution of the first reagent along the pathway.

13. The device of claim 1 wherein the second reagent is configured to speed up the temporal distribution of the first reagent along the pathway and/or streamline the spatial distribution of the first reagent along the pathway.

14. The device of claim 1 further comprising a third reagent placed in a ring about the first reagent on the pathway.