Data processing system and methods
First Claim
Patent Images
1. A method of tracking the location of features of a specimen across a set of images of the specimen captured at different reference points, the set of images comprising a plurality of cycles of images, each cycle comprising different images, each image comprising signals having a single color, wherein the signal color is different for each of the different images, said method comprising the steps of:
- (a) selecting a subset of images, wherein the images of the subset depict signals corresponding to features of the specimen, and wherein the images of the subset are captured at different reference points;
(b) selecting a primary image from the subset of images, comprising;
(i) selecting the different color images from a single cycle within the subset of images,(ii) merging signals from at least two different color images of each cycle into a candidate primary image for said cycle,(iii) repeating steps (i) and (ii), thereby forming a plurality of candidate primary images, and(iv) selecting the candidate primary image from among the candidate primary images that includes the most signals, thereby obtaining a primary image;
(c) registering the signals depicted in the images of the subset of images with the signals depicted in the primary image, so as to determine the location of the signals depicted in the images with respect to each other, thereby producing signal clumps;
(d) selecting a signal from each of said signal clumps, thereby forming a template that permits the identification of the locations of features of said specimen; and
(e) registering remaining images in said set of images with said template.
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Abstract
Methods and systems for analysis of image data generated from various reference points. Particularly, the methods and systems provided are useful for real time analysis of image and sequence data generated during DNA sequencing methodologies.
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Citations
20 Claims
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1. A method of tracking the location of features of a specimen across a set of images of the specimen captured at different reference points, the set of images comprising a plurality of cycles of images, each cycle comprising different images, each image comprising signals having a single color, wherein the signal color is different for each of the different images, said method comprising the steps of:
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(a) selecting a subset of images, wherein the images of the subset depict signals corresponding to features of the specimen, and wherein the images of the subset are captured at different reference points; (b) selecting a primary image from the subset of images, comprising; (i) selecting the different color images from a single cycle within the subset of images, (ii) merging signals from at least two different color images of each cycle into a candidate primary image for said cycle, (iii) repeating steps (i) and (ii), thereby forming a plurality of candidate primary images, and (iv) selecting the candidate primary image from among the candidate primary images that includes the most signals, thereby obtaining a primary image; (c) registering the signals depicted in the images of the subset of images with the signals depicted in the primary image, so as to determine the location of the signals depicted in the images with respect to each other, thereby producing signal clumps; (d) selecting a signal from each of said signal clumps, thereby forming a template that permits the identification of the locations of features of said specimen; and (e) registering remaining images in said set of images with said template. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification