Method and apparatus for separating a material
First Claim
1. A method for isolating at least two fractions of a multiple component material comprising:
- loading the multiple component material into a separation chamber of a separation device between a valve mounted at a fixed position in the separation chamber and a first end of the device, the first end is opposite to a second end and a sidewall extends between the first end and the second end to define the separation chamber having an interior volume wherein the valve includes a screen, a valve activation member, and a seal, and wherein each of the screen, the valve activation member, and the seal define a through hole at an axial center thereof;
wherein the through hole is in fluid communication with apertures at an end of the valve activation member proximate to the second end; and
wherein an extraction tube is seated in the through hole and is connected to an extraction port of the separation device;
centrifuging the separation device such that the valve moves to an open position in response to gravitational forces exerted on the device to permit a first fraction of the multiple component material of a first density to pass through the valve toward the second end;
ceasing centrifugation of the separation device to permit the valve to move to a closed position, thus isolating the first fraction of the first density between the valve and the second end and isolating a second fraction of a second density that is less dense than the first density between the valve and the first end; and
withdrawing at least one of the first fraction and the second fraction from the separation chamber for use in a subsequent procedure.
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Accused Products
Abstract
A separation system for separating a multiple component material into at least two fractions. The separation system includes a separation device having a first end, a second end opposite to the first end, and a sidewall that extends between the first end and the second end to define a separation chamber having an interior volume. The system also includes a valve moveable between an open position and a closed position, the valve is mounted at a fixed location within the separation chamber at a position that is closer to the second end than to the first end and is spaced apart from the second end, the valve is operable to isolate a first fraction of the multiple component material having a first density on a first side of the valve from a second fraction having a second density on a second side of the valve that is opposite to the first side.
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Citations
20 Claims
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1. A method for isolating at least two fractions of a multiple component material comprising:
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loading the multiple component material into a separation chamber of a separation device between a valve mounted at a fixed position in the separation chamber and a first end of the device, the first end is opposite to a second end and a sidewall extends between the first end and the second end to define the separation chamber having an interior volume wherein the valve includes a screen, a valve activation member, and a seal, and wherein each of the screen, the valve activation member, and the seal define a through hole at an axial center thereof;
wherein the through hole is in fluid communication with apertures at an end of the valve activation member proximate to the second end; and
wherein an extraction tube is seated in the through hole and is connected to an extraction port of the separation device;centrifuging the separation device such that the valve moves to an open position in response to gravitational forces exerted on the device to permit a first fraction of the multiple component material of a first density to pass through the valve toward the second end; ceasing centrifugation of the separation device to permit the valve to move to a closed position, thus isolating the first fraction of the first density between the valve and the second end and isolating a second fraction of a second density that is less dense than the first density between the valve and the first end; and withdrawing at least one of the first fraction and the second fraction from the separation chamber for use in a subsequent procedure. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method for isolating at least two fractions from adipose tissue comprising:
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loading the adipose tissue into a separation chamber of a separation device between a valve mounted at a fixed position in the separation chamber and a first end of the device, the first end is opposite to a second end and a sidewall extends between the first end and the second end to define the separation chamber having an interior volume, wherein the valve includes a screen, a valve activation member, and a seal, and wherein each of the screen, the valve activation member, and the seal define a through hole at an axial center thereof, wherein the through hole is in fluid communication with apertures at an end of the valve activation member proximate to the second end, and wherein an extraction tube is seated in the through hole and is connected to an extraction portion of the separation device; centrifuging the separation device at about 1500×
g to about 3000×
g for about 15 minutes such that the valve moves to an open position in response to gravitational forces exerted on the device to permit a first dense fraction of the adipose tissue comprising cellular material to pass through the valve toward the second end;ceasing centrifugation of the separation device to permit the valve to move to a closed position, thus isolating the first dense fraction between the valve and the second end and isolating a second less dense fraction comprising purified fat between the valve and the first end, wherein a tumescent fluid layer is isolated on both sides of the valve; and withdrawing at least one of the cellular material and the purified fat from the separation chamber for use in a subsequent procedure. - View Dependent Claims (12, 13, 14, 15, 16)
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17. A method for isolating at least two fractions from adipose tissue comprising:
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extracting adipose tissue from the patient and simultaneously loading the tissue directly into a separation device using a vacuum pump, wherein the adipose tissue is loaded into a separation chamber of the separation device between a valve mounted at a fixed position in the separation chamber and a first end of the device, the first end is opposite to a second end and a sidewall extends between the first end and the second end to define the separation chamber having an interior volume wherein the valve includes a screen, a valve activation member, and a seal, and wherein each of the screen, the valve activation member, and the seal define a through hole at an axial center thereof;
wherein the through hole is in fluid communication with apertures at an end of the valve activation member proximate to the second end; and
wherein an extraction tube is seated in the through hole and is connected to an extraction port of the separation device;centrifuging the separation device such that the valve moves to an open position in response to gravitational forces exerted on the device to permit a first dense fraction of the adipose tissue comprising cellular material to pass through the valve toward the second end; ceasing centrifugation of the separation device to permit the valve to move to a closed position, thus isolating the first dense fraction between the valve and the second end and isolating a second less dense fraction comprising purified fat between the valve and the first end, wherein a tumescent fluid layer is isolated on both sides of the valve; withdrawing at least one of the cellular material and the purified fat from the separation chamber; and administering the cellular material or the purified fat to the patient. - View Dependent Claims (18, 19, 20)
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Specification