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Methods, compositions, and kits for detecting allelic variants

  • US 9,534,255 B2
  • Filed: 12/17/2009
  • Issued: 01/03/2017
  • Est. Priority Date: 12/17/2008
  • Status: Active Grant
First Claim
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1. A composition comprising:

  • a) an allele-specific primer that is complementary to a target sequence comprising a first allelic variant;

    b) an allele-specific blocker probe that does not comprise a label and is complementary to said target sequence comprising a second allelic variant, wherein said second allelic variant corresponds to a position in said target sequence that is at the same loci as said first allelic variant in said target sequence, and wherein said allele-specific blocker probe comprises a minor groove binder (MGB) at the 3′

    end that is non-extendable;

    c) a locus-specific primer that is complementary to a region of the target sequence that is 3′

    from the first allelic variant and on the opposite strand; and

    d) a locus-specific detector probe wherein the detector probe is a 5′

    nuclease probe and has two labels.

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