Biosensors and methods of preparing same

• US 9,535,028 B2
• Filed: 06/22/2015
• Issued: 01/03/2017
• Est. Priority Date: 06/08/2005
• Status: Expired due to Fees

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Petyt, Adrian, Bates, Matthew, Reid, Pamela, Bull, Andrew J., Garner, Jeff
• Primary Examiner(s): Hendricks, Keith
• Assistant Examiner(s): CARLSON, KOURTNEY SALZMAN
• Application Number:
  US14/746,393
• Publication Number:
  US 20150355130A1
• Time in Patent Office: 561 Days
• Field of Search: 20440301-40314, 205/777.5
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: G01N 27/327 Biochemical electrodes , e.g. electrical or mechanical details for in vitro measurements
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10T 156/1052 with cutting, punching, tearing or severing

Abstract

A biosensor strip having a low profile for reducing the volume of liquid sample needed to perform an assay. In one embodiment, the biosensor strip includes an electrode support; an electrode arrangement on the electrode support; a cover; a sample chamber; and an incompressible element placed between the cover and the electrode support, the incompressible element providing an opening in at least one side or in the distal end of the sample chamber to provide at least one vent in the sample chamber. In another embodiment, the biosensor strip has an electrode support; an electrode arrangement on the electrode support; a cover; and a sample chamber, the cover having a plurality of openings formed therein, at least one of the openings in register with the sample chamber. The invention further includes methods for preparing such a biosensor strips in a continuous manner.

Claims

1. A glucose sensor, comprising:

a substrate;

an electrode positioned on the substrate, the electrode comprising a glucose responsive-enzyme and a mediator disposed thereon;

a cover;

a spacer layer positioned between the substrate and the cover and forming a sample chamber there between, wherein a portion of the electrode is present in the sample chamber, the sample chamber comprising a proximal end where a sample is introduced into the sample chamber and a distal end toward which the sample flows; and

an incompressible element placed between the cover and the spacer layer, said incompressible element positioned only at or near the distal end of the sample chamber and providing at least one vent in the sample chamber at a lateral side of the glucose sensor.

2. The glucose sensor of claim 1, wherein the at least one vent is in at least one side of the sample chamber.

3. The glucose sensor of claim 1, wherein the at least one vent is in a distal end of the sample chamber.

4. The glucose sensor of claim 1, wherein the incompressible element is selected from the group consisting of at least one filament, at least one thread, at least one ribbon, and at least one tape.

5. The glucose sensor of claim 1, further comprising at least one layer of mesh is interposed between the cover and the electrode arrangement.

6. The glucose sensor of claim 5, wherein the at least one layer of mesh comprises polyester.

7. The glucose sensor of claim 5, wherein the at least one layer of mesh comprises a surfactant thereon.

8. The glucose sensor of claim 1, wherein the cover includes a backing having a layer of adhesive on one major surface thereof.

9. The glucose sensor of claim 1, wherein the volume of the sample chamber is no more than about 1 microliter.

10. The glucose sensor of claim 1, wherein the volume of the sample chamber is no more than about 0.5 microliters.

11. The glucose sensor of claim 1, wherein the volume of the sample chamber is no more than about 0.3 microliters.

12. The glucose sensor of claim 1, wherein the volume of the sample chamber is no more than about 0.1 microliter.

13. The glucose sensor of claim 1, configured to determine the concentration of the glucose in about 1-10 seconds.

14. The glucose sensor of claim 1, configured to determine the concentration of the glucose in about 3-5 seconds.

15. The glucose sensor of claim 1, configured to determine the concentration of the glucose in about 1-3 seconds.

16. The glucose sensor of claim 1, wherein the incompressible element is comprised of a hydrophobic material.

17. The glucose sensor of claim 1, wherein the glucose sensor comprises a first side edge extending between the proximal end and the distal end and a second side edge extending between the proximal end and the distal end, wherein the incompressible element extends from the first side edge to the second side edge.

18. The glucose sensor of claim 1, wherein the incompressible element is a monofilament, a thread, a ribbon, or a tape.