Dual-functional nonfouling surfaces comprising target binding partner covalently coupled to polymer attached to substrate

• US 9,535,062 B2
• Filed: 08/27/2014
• Issued: 01/03/2017
• Est. Priority Date: 12/29/2006
• Status: Active Grant

• Current Assignee: University of Washington
• Original Assignee: University of Washington
• Inventors: Jiang, Shaoyi, Zhang, Zheng, Chen, Shengfu, Vaisocherova, Hana
• Primary Examiner(s): JONES JR., ROBERT STOCKTON
• Application Number:
  US14/470,775
• Publication Number:
  US 20140370567A1
• Time in Patent Office: 860 Days
• US Class Current: 1/1
• CPC Class Codes: C08F 122/22 Esters containing nitrogen
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/545 Synthetic resin

Abstract

Dual-functional nonfouling surfaces and materials, methods for making dual-functional nonfouling surfaces and materials, and devices that include dual-functional nonfouling surfaces and materials. The dual-functional surfaces are nonfouling surfaces that resist non-specific protein adsorption and cell adhesion. The dual-functional surfaces and materials include covalently coupled biomolecules (e.g., target binding partners) that impart specific biological activity thereto. The surfaces and materials are useful in medical diagnostics, biomaterials and bioprocessing, tissue engineering, and drug delivery.

Claims

1. A block copolymer having a plurality of covalently coupled target binding partners having affinity toward a target molecule, the block copolymer having a first hydrophilic block and a second hydrophobic block, wherein the hydrophilic block comprises a plurality of carboxylic acid groups and a plurality of positive charged groups, wherein the hydrophilic block is substantially electronically neutral, and wherein the target binding partners are selected from the group consisting of enzymes, antibodies, protein-based antigens, receptors, polysaccharide ligands, and oligosaccharides ligands.

2. The block copolymer of claim 1, wherein the hydrophilic block is a poly(carboxybetaine).

3. The block copolymer of claim 2, wherein the poly(carboxybetaine) is prepared from one or more monomers selected from the group consisting of carboxybetaine acrylates, carboxybetaine acrylamides, carboxybetaine vinyl compounds, carboxybetaine epoxides, and mixture thereof.

4. The block copolymer of claim 1, wherein the block copolymer is in the form of a microparticle or a nanoparticle.

5. The block copolymer of claim 1, wherein the hydrophobic block is selected from the group consisting of poly(propylene oxide), polymethacrylates, polyacrylates, polyacrylamides, polyesters, polyethers, polyurethanes, and polyamides.

6. The block copolymer of claim 1, wherein the hydrophobic block is a polyester.

7. The block copolymer of claim 1, wherein the hydrophilic block is a zwitterionic polymer block.

8. The block copolymer of claim 1, wherein the poly (carboxybetaine) is prepared from 2-carboxy-N,N-dimethyl-N-(2′

-methacryloyloxyethyl) ethanaminium inner salt.