High-sensitivity nanoscale wire sensors

• US 9,535,063 B2
• Filed: 09/18/2013
• Issued: 01/03/2017
• Est. Priority Date: 11/22/2006
• Status: Expired due to Fees

• Current Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Original Assignee: President and Fellows of Harvard College (Harvard Corporation)
• Inventors: Lieber, Charles M., Gao, Xuan, Zheng, Gengfeng
• Primary Examiner(s): WHALEN, DANIEL B
• Application Number:
  US14/030,170
• Publication Number:
  US 20140080139A1
• Time in Patent Office: 1,203 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 27/4146 involving nanosized elements, e.g. nanotubes, nanowires
• CPC Class Codes: G01N 33/552 Glass or silica

Abstract

One aspect of the invention provides a nanoscale wire that has improved sensitivity, for example, as the carrier concentration in the wire is controlled by an external gate voltage. In one set of embodiments, the nanoscale wire has a Debye screening length that is greater than the average cross-sectional dimension of the nanoscale wire when the nanoscale wire is exposed to a solution suspected of containing an analyte. In certain instances, the Debye screening length associated with the carriers inside nanoscale wire may be adjusted by adjusting the voltage, for example, a gate voltage applied to an FET structure. In some cases, the nanoscale wire can be operated under conditions where the carriers in the nanoscale wire are depleted and the nanoscale wire has a conductance that is not linearly proportional to the voltage applied to the nanoscale wire sensor device, for example, via a gate electrode.

Claims

1. A method, comprising:

exposing a nanoscale wire, having a reaction entity immobilized relative thereto, to a solution suspected of containing an analyte that the reaction entity is able to bind,

wherein the nanoscale wire has a Debye screening length, when the nanoscale wire is placed in the solution, that is greater than the average cross-sectional dimension of the nanoscale wire.

3. The method of claim 1 further comprising

determining a change in charge of the analyte of less than about 10⁻

17 coulombs.

4. The method of claim 3, wherein the change in charge of the analyte is less than about 20 electron charges.

5. The method of claim 3, wherein the change in charge of the analyte is less than about 10 electron charges.

6. The method of claim 1, wherein the reaction entity comprises a nucleic acid.

7. The method of claim 1, wherein the reaction entity comprises a protein.

8. The method of claim 1, wherein the reaction entity comprises an enzyme.

9. The method of claim 1, wherein the reaction entity comprises an antibody.

10. The method of claim 1, wherein the reaction entity is covalently immobilized to the nanoscale wire.

11. The method of claim 1, wherein the reaction entity is immobilized to the nanoscale wire via a linker.

12. The method of claim 1, wherein the reaction entity is positioned within 5 nanometers of the nanowire.

13. The method of claim 1, wherein the reaction entity is positioned within 3 nanometers of the nanowire.

14. The method of claim 1, wherein the reaction entity is positioned within 1 nanometer of the nanowire.

15. The method of claim 3, wherein the nanoscale wire is a semiconductor nanowire.

16. The method of claim 15, wherein the semiconductor nanowire is a silicon nanowire.

17. The method of claim 1, wherein the reaction entity specifically binds the analyte.

18. The method of claim 1, wherein the reaction entity nonspecifically binds the analyte.

19. The method of claim 3, wherein the nanoscale wire is contained in the solution.

20. The method of claim 1, wherein the solution has an ionic strength of less than about 10 millimolar.

2. A method, comprising:

exposing a nanoscale wire, having a reaction entity immobilized relative thereto, to a solution suspected of containing an analyte that the reaction entity is able to bind; and

operating the nanoscale wire under conditions wherein the nanoscale wire has a conductance that is not linearly proportional to voltage applied to the nanoscale wire.