Root evaluation
First Claim
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1. A method for evaluating roots of a potted plant in a non-destructive manner to identify a plant possessing a trait of interest, comprising:
- (i) providing a potted plant grown in a particulate, non-transparent growing medium in a substantially transparent container for imaging;
(ii) evaluating roots of the potted plant through the transparent container and the particulate, non-transparent growing medium by using a camera that can output two-dimensional images in a digital format;
(iii) collecting digital images of the roots and storing information from the digital images in a computer; and
(iv) measuring root traits by analyzing the information with algorithms to identify a plant possessing a trait of interest,wherein the roots of the potted plant are evaluated in a non-destructive manner to the plant and the roots, andwherein the potted plant is retrieved from an original location for imaging and returned to its original location or to another location after imaging.
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Abstract
A method is provided for evaluating plant roots, comprising (i) growing a plant in a substantially transparent container charged with a particulate, non-transparent growing medium; and (ii) evaluating plant roots through the transparent container by digital imaging. An apparatus for evaluating plant roots in a high throughput manner is also provided.
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Citations
34 Claims
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1. A method for evaluating roots of a potted plant in a non-destructive manner to identify a plant possessing a trait of interest, comprising:
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(i) providing a potted plant grown in a particulate, non-transparent growing medium in a substantially transparent container for imaging; (ii) evaluating roots of the potted plant through the transparent container and the particulate, non-transparent growing medium by using a camera that can output two-dimensional images in a digital format; (iii) collecting digital images of the roots and storing information from the digital images in a computer; and (iv) measuring root traits by analyzing the information with algorithms to identify a plant possessing a trait of interest, wherein the roots of the potted plant are evaluated in a non-destructive manner to the plant and the roots, and wherein the potted plant is retrieved from an original location for imaging and returned to its original location or to another location after imaging. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method for evaluating roots of a potted plant in a non-destructive manner to identify a plant possessing a trait of interest, comprising:
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(i) providing a potted plant grown in a particulate, non-transparent growing medium comprising soil in a substantially transparent container; (ii) placing the potted plant in a position for image capturing with a camera that can output two-dimensional images in a digital format; (iii) digital imaging roots of the potted plant through the transparent container and the particulate, non-transparent growing medium; (iv) collecting digital images of the roots and storing information from the digital images in a computer; and (v) measuring root traits by analyzing the information with algorithms to identify a plant possessing a trait of interest wherein the roots of the potted plant are evaluated in a non-destructive manner to the plant and the roots, and wherein the potted plant is retrieved from an original location for imaging and returned to its original location or to another location after imaging. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. A method for evaluating roots of a potted plant in a non-destructive manner to identify a plant possessing a trait of interest, comprising:
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(i) providing a potted plant grown in a particulate, non-transparent growing medium comprising soil in a container for imaging, wherein at least the bottom of said container is substantially transparent; (ii) placing the potted plant into a water basin, wherein said water basin has a transparent bottom and is placed above a camera that can output images in a digital format; (iii) digital imaging roots of the potted plant through the bottom of the container; (iv) collecting digital images of the roots and storing information from the digital images in a computer; and (v) measuring root traits by analyzing the information with algorithms to identify a plant possessing a trait of interest, wherein the roots of the potted plant are evaluated in a non-destructive manner to the plant and the roots, and wherein the potted plant is retrieved from an original location for imaging and returned to its original location or to another location after imaging. - View Dependent Claims (28, 29, 30, 31, 32, 33, 34)
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Specification