Stacked herbicide tolerance event 8264.44.06.1, related transgenic soybean lines, and detection thereof

• US 9,540,655 B2
• Filed: 12/02/2011
• Issued: 01/10/2017
• Est. Priority Date: 12/03/2010
• Status: Active Grant

• Current Assignee: Dow Agrosciences LLC (Corteva, Inc.), MS Technologies LLC
• Original Assignee: Dow Agrosciences LLC (Corteva, Inc.), MS Technologies LLC
• Inventors: Cui, Yunxing, Hoffman, Thomas, Zhou, Ning, Novak, Stephen N., Colon, Julissa, Parkhurst, Dawn, Toledo, Sandra, Wright, Terry, Russell, Sean, Held, Bruce, Sekar, Vaithilingam
• Primary Examiner(s): Visone, Lee A
• Assistant Examiner(s): Weihua, Fan
• Application Number:
  US13/991,246
• Publication Number:
  US 20140041083A1
• Time in Patent Office: 1,866 Days
• US Class Current: 1/1
• CPC Class Codes: A01N 39/04 Aryloxy-acetic acids; Derivatives thereof
• CPC Class Codes: C12N 15/8241 Phenotypically and genetically modified plants via recombinant DNA technology
• CPC Class Codes: C12N 15/8274 for herbicide resistance
• CPC Class Codes: C12N 15/8275 Glyphosate
• CPC Class Codes: C12N 9/1092 3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
• CPC Class Codes: C12N 9/93 Ligases (6)
• CPC Class Codes: C12Q 1/6895 for plants, fungi or algae
• CPC Class Codes: C12Q 2600/13 Plant traits
• CPC Class Codes: C12Q 2600/156 Polymorphic or mutational markers
• CPC Class Codes: C12Q 2600/16 Primer sets for multiplex assays
• CPC Class Codes: C12Y 205/01019 3-Phosphoshikimate 1-carboxyvinyltransferase (2.5.1.19), i.e. 5-enolpyruvylshikimate-3-phosphate synthase
• CPC Class Codes: C12Y 604/01002 Acetyl-CoA carboxylase (6.4.1.2)

Abstract

This invention relates in part to soybean event pDAB8264.44.06.1 and includes a novel expression cassettes and transgenic inserts comprising multiple traits conferring resistance to glyphosate, aryloxyalkanoate, and glufosinate herbicides. This invention also relates in part to methods of controlling resistant weeds, plant breeding and herbicide tolerant plants. In some embodiments, the event sequence can be “stacked” with other traits, including, for example, other herbicide tolerance gene(s) and/or insect-inhibitory proteins. This invention further relates in part to endpoint TaqMan PCR assays for the detection of Event pDAB8264.44.06.1 in soybeans and related plant material. Some embodiments can perform high throughput zygosity analysis of plant material and other embodiments can be used to uniquely identify the zygosity of and breed soybean lines comprising the event of the subject invention. Kits and conditions useful in conducting these assays are also provided.

Claims

1. A transgenic soybean plant cell comprising a genome comprising SEQ ID NO:

27 wherein said SEQ ID NO;

27 comprises a first sequence represented by SEQ ID NO;

16 and a second sequence represented by SEQ ID NO;

17, wherein said first sequence spans the 5′

integration junction of said polynucleotide segment and said second sequence spans the 3′

integration junction of said polynucleotide segment.

3. A soybean seed comprising the transgenic soybean plant cell of claim 1, said seed comprising said polynucleotide segment.

6. A transgenic soybean plant comprising a plurality of cells of claim 1.

7. The transgenic soybean plant of claim 6, said plurality of cells further comprising an insect resistance gene.

8. The transgenic soybean plant of claim 6 wherein the plant is resistant to at least one herbicide selected from the group consisting of a phenoxyacetic acid herbicide, a phenoxybutanoic acid herbicide, a pyridyloxyalkanoic acid herbicide, a glyphosate herbicide, and a glufosinate herbicide.

12. The transgenic soybean plant of claim 6, wherein the plant is from Glycine max.

13. A soybean plant produced by growing the soybean seed of claim 3, said plant comprising said polynucleotide segment.

14. A part of the soybean plant of claim 13 wherein said part is selected from the group consisting of pollen, an ovule, a flower, a shoot, a root, and a leaf, said part comprising said polynucleotide segment.

15. A method of controlling weeds in a field, said method comprising applying at least one herbicide selected from the group consisting of a 2,4-dichlorophenoxyacetic acid, a glyphosate, and a glufosinate to the field, and planting a seed of claim 3.

2. A soybean seed comprising a genome comprising Event pDAB8264.44.06.1, wherein said soybean seed is present in representative seed deposited with American Type Culture Collection (ATCC) under Accession No. PTA-11336.

4. A soybean plant produced by growing the seed of claim 2, said plant comprising said Event pDAB8264.44.06.1.

5. A progeny plant of the soybean plant of claim 4, said progeny plant comprising said Event pDAB8264.44.06.1.

9. A part of the soybean plant of claim 4 wherein said part is selected from the group consisting of pollen, an ovule, a flower, a shoot, a root, and a leaf, said part comprising said Event pDAB8264.44.06.1.

11. A method of controlling weeds in a field, said method comprising applying a herbicide selected from the group consisting of a phenoxyacetic acid, a phenoxybutanoic acid, a pyridyloxyalkanoic acid, a glyphosate, and a glufosinate to the field, and planting a seed of claim 2 in the field within 14 days of applying the herbicide.

10. A plant cell comprising a genome comprising Event pDAB8264.44.06.1 as present in representative seeds deposited with American Type Culture Collection (ATCC) under Accession No. PTA-11336.