Process for producing ethanol from textile cotton

• US 9,540,665 B2
• Filed: 12/04/2009
• Issued: 01/10/2017
• Est. Priority Date: 12/05/2008
• Status: Expired due to Fees

• Current Assignee: valagro carbone renouvelable poitou charentes
• Original Assignee: valagro carbone renouvelable poitou charentes
• Inventors: Barbier, Jacques, Bataille, Frederic, Briand, Alexandre
• Primary Examiner(s): ROBINSON, HOPE A
• Application Number:
  US13/133,073
• Publication Number:
  US 20110236945A1
• Time in Patent Office: 2,594 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C12P 7/08 produced as by-product or from waste or cellulosic material substrate
• CPC Class Codes: C12P 7/10 substrate containing cellulosic material
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

A recycled textile cotton used for producing ethanol by the execution of a process that includes a step of pretreatment of textile cotton by pulping in an extruder, which may or may not involve a grinding step prior to pulping in the extruder, a step of enzymatic hydrolysis, a step of filtration(s), and a step of fermentation.

Claims

1. A process for the production of ethanol from textile cotton, comprising the steps of:

a) Pre-treating textile cotton, said pre-treating consisting of a mechanical process selected from the group consisting of;

(1) pulping a mixture of textile cotton and water in a twin-screw extruder at a temperature of between 60°

C. and 180°

C., wherein the water represents between 200% and 450% of the mass of the textile cotton,

and

(2) (i) grinding textile cotton,

(ii) pulping a mixture of the ground textile cotton obtained from (i) with water in a twin-screw extruder at a temperature of between 60°

C. and 180°

C., wherein the water represents between 200% and 450% of the mass of the ground textile cotton;

b) Performing enzymatic hydrolysis of the pre-treated textile cotton and water mixture to form a medium comprising a sugary juice, enzymes used in said enzymatic hydrolysis, non-cellulose compounds, non-degraded cellulose, and dye from the textile cotton;

c) Performing filtration of the medium to separate the sugary juice from the enzymes, non-cellulose compounds, non-degraded cellulose and dye;

d) Fermenting the sugary juice with yeast, and

e) Obtaining ethanol from step d).

2. The process for the production of ethanol from textile cotton of claim 1, further comprising a step of performing discoloration to remove the dye before step d).

3. The process for the production of ethanol from textile cotton of claim 1, wherein the filtration of step c) comprises:

(i) Filtration to clarify the medium by removing the non-cellulose compounds and the non-degraded cellulose from the medium;

(ii) Ultrafiltration of the clarified medium to concentrate and remove the enzymes and to obtain a permeate comprising the sugary juice and the dye;

(iii) Reverse Osmosis to concentrate the permeate;

(iv) Discoloration to remove dye from the permeate to obtain the sugary juice;

(v) Sterile filtration of the sugary juice; and

(vi) Cooling.

4. The process for the production of ethanol from textile cotton of claim 1, wherein the ethanol is obtained in step e) by distillation or dehydration.

5. The process for the production of ethanol from textile cotton of claim 4, wherein the distillation or dehydration is executed using a membrane by pervaporation.

6. The process for the production of ethanol from textile cotton of claim 2,

wherein the process further comprises a step of performing microfiltration for recycling the yeasts used in step d);

wherein the ethanol is obtained in step e) by distillation or dehydration;

wherein the enzymatic hydrolysis in step b) comprises using an enzymatic cocktail that consists of cellulases and β

-glucosidase;

wherein the filtration of step c)comprises performing;

(i) Filtration to clarify the medium by removing the non-cellulose compounds and the non-degraded cellulose from the medium,

(ii) Ultrafiltration of the clarified medium to concentrate and to remove the cellulases and β

-glucosidase for recycling and to obtain a permeate comprising the sugary juice and the dye,

(iii) Reverse osmosis for concentrating the permeate,

(iv) Discoloration to remove the dye from the permeate to obtain a sugary juice,

(v) Sterile filtration of the sugary juice, and

(vi) Cooling; and

wherein step d) is performed in a fermenter between 30°

C. and 37°

C., between 7 hours and 24 hours, at a pH of between 3.8 and 5.0, with Saccharomyces cerevisiae as the yeast.

7. The process for the production of ethanol from textile cotton of claim 2, wherein the ethanol is obtained in step e) by distillation or dehydration.

8. The process for the production of ethanol from textile cotton of claim 3, wherein the ethanol is obtained in step e) by distillation or dehydration.

9. The process for the production of ethanol from textile cotton of claim 3,

wherein the process further comprises a step of performing microfiltration for recycling the yeasts used in step d);

wherein the ethanol is obtained in step e) by distillation or dehydration;

wherein the enzymatic hydrolysis in step b) comprises using an enzymatic cocktail that consists of cellulases and β

-glucosidase; and

wherein step d) is performed in a fermenter between 30°

C. and 37°

C., between 7 hours and 24 hours, at a pH of between 3.8 and 5.0, with Saccharomyces cerevisiae as the yeast.

10. The process for the production of ethanol from textile cotton of claim 4

wherein the process further comprises a step of performing microfiltration for recycling the yeasts used in step d);

wherein the enzymatic hydrolysis in step b) comprises using an enzymatic cocktail that consists of cellulases and β

-glucosidase;

wherein the filtration of step c)comprises performing;

(i) Filtration to clarify the medium by removing the non-cellulose compounds and the non-degraded cellulose from the medium,

(ii) Ultrafiltration of the clarified medium to concentrate and to remove the cellulases and

β

-glucosidase for recycling and to obtain a permeate comprising the sugary juice and the dye,

(iii) Reverse osmosis for concentrating the permeate,

(iv) Discoloration to remove the dye from the permeate to obtain a sugary juice,

(v) Sterile filtration of the sugary juice, and

(vi) Cooling;

wherein step d) is performed in a fermenter between 30°

C. and 37°

C., between 7 hours and 24 hours, at a pH of between 3.8 and 5.0, with Saccharomyces cerevisiae as the yeast.

11. The process for the production of ethanol from textile cotton of claim 1, wherein the pretreating consists of the mechanical process of:

(i)grinding textile cotton, and

(ii) pulping a mixture of the ground textile cotton obtained from (i) and water in a twin-screw extruder at a temperature of between 60°

C. and 180°

C., wherein the water represents between 200% and 450% of the mass of the ground textile cotton.

12. A process for the production of ethanol from textile cotton, comprising the steps of:

pretreating textile cotton, said pretreating consisting of a mechanical process of;

(i) grinding textile cotton to form a ground textile cotton and

(ii) pulping the ground textile cotton obtained in (i) with water that is between 200% and 450% of the mass of ground textile cotton in a twin-screw extruder at a temperature of between 60°

C. and 180°

C.;

preparing a reaction medium for enzymatic hydrolysis in which the pre-treated textile cotton and the water are combined with enzymes for hydrolysis;

filtering the medium after enzymatic hydrolysis to remove non-cellulose and non-degraded cellulose from the medium thereby obtaining a clarified medium;

subjecting the clarified medium to ultrafiltration to recover a concentrate and a permeate, the concentrate being enzymes of the hydrolysis and the permeate being a sugary juice;

subjecting the sugary juice to reverse osmosis to concentrate the sugary juice;

subjecting the concentrated sugary juice to sterile filtration to obtain a sterile concentrated sugary juice;

subjecting the sterile concentrated sugary juice to fermentation with yeast in a fermenter,

recovering the yeast used in the fermenting by microfiltration; and

distilling or dehydrating the fermented sugary juice to produce ethanol, wherein

at least one of the recovered concentrate of enzymes or the recovered yeast is recycled in the enzymatic hydrolysis step or the fermentation step, respectively.

13. The method according to claim 12, wherein the enzymes used in the enzymatic hydrolysis consist of cellulases and β

-glucosidase.

14. The method according to claim 12, wherein the yeast is Saccharomyces cerevisiae, and fermentation is carried out between 30°

C. and 37°

C., between 7 hours and 24 hours, at a pH of between 3.8 and 5.0.

15. The method according to claim 12, wherein the sterile concentrated sugary juice is cooled prior to fermentation.

16. The method according to claim 12, wherein discoloration is performed prior to fermentation to remove dye originating from the textile cotton.