Methods of diagnosing increased risk of developing MRSA
First Claim
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1. A method comprising the steps of:
- obtaining a biological sample from a human subject, wherein said biological sample comprises genomic DNA,analyzing the genomic DNA and detecting the presence of two copies of SEQ ID NO;
1 in the sample,predicting that the subject has an increased risk of developing a MRSA infection, andadministering to the human subject anti MRSA antibiotics.
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Abstract
A method for diagnosing increased risk of developing Methicillin-resistant Staphylococcus aureus (MRSA) hospital-acquired (HA-MRSA) or community-acquired MRSA (CA-MRSA) which includes obtaining a biological sample from a subject, detecting in the sample a single nucleotide polymorphism (SNP) in the FAM129B gene at position 17 of SEQ ID NO 1, and comparing the nucleotide at position 17 of SEQ ID NO. 1 in the sample with the nucleotide at position 17 in SEQ ID NO. 1, wherein an adenine at position 17 of SEQ ID NO. 1 in the sample indicates an increased risk of developing MRSA or CA-MRSA in the subject.
12 Citations
11 Claims
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1. A method comprising the steps of:
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obtaining a biological sample from a human subject, wherein said biological sample comprises genomic DNA, analyzing the genomic DNA and detecting the presence of two copies of SEQ ID NO;
1 in the sample,predicting that the subject has an increased risk of developing a MRSA infection, and administering to the human subject anti MRSA antibiotics.
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2. The method of claim 1, wherein the detecting step comprising contacting the genomic DNA with an oligonucleotide probe that comprises a locus corresponding to position 17 of SEQ ID NO:
- 1.
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3. The method of claim 2, wherein the detecting step further comprise sequencing the oligonucleotide from the biological sample.
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4. The method of claim 3, wherein the detecting step further comprises amplifying the nucleic acid that comprises instant SEQ ID NO:
- 1 from the genomic DNA.
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5. The method of claim 4, wherein the amplifying uses at least one oligonucleotide primer.
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6. The method of claim 5, wherein the oligonucleotide primer comprises DNA.
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7. The method of claim 1, wherein the treating further comprises administering to the patient decolonization treatments.
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8. The method of claim 7, wherein the patient is administered more than one course of anti MRSA antibiotics and is put on high infection alert for future surgeries.
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9. The method of claim 1, wherein the human subject is preparing for surgery and further comprising taking additional steps to prevent infection.
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10. A method comprising the steps of:
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obtaining a biological sample from a human subject, wherein said biological sample comprises genomic DNA, analyzing the genomic DNA and detecting the presence of two copies of SEQ ID NO;
2 in the sample,predicting that the subject has a decreased risk of developing a MRSA infection, and treating the subject with incision and drainage in the absence of antibiotics.
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11. A method comprising the steps of:
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obtaining a biological sample from a human subject, wherein said biological sample comprises genomic DNA, analyzing the genomic DNA and detecting the presence of two copies of SEQ ID NO;
2 in the sample,predicting that the subject has a decreased risk of developing a MRSA infection, and treating the subject with incision and drainage and a common antibiotic.
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Specification