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Capture, purification, and release of biological substances using a surface coating

  • US 9,541,480 B2
  • Filed: 06/28/2012
  • Issued: 01/10/2017
  • Est. Priority Date: 06/29/2011
  • Status: Active Grant
First Claim
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1. A method comprising:

  • (a) contacting a biological sample comprising rare cells and non-specific cells to a non-fouling lipid layer of a microfluidic device, the microfluidic device comprising an antibody which selectively binds to rare cells, and wherein the non-fouling lipid layer is non-covalently associated with the antibody;

    (b) binding the rare cells to the antibody;

    (c) washing the non-fouling lipid layer with a liquid, wherein the non-specific cells in contact with the non-fouling lipid layer are released from the non-fouling lipid layer; and

    (d) flowing a liquid comprising bubbles through a channel of the microfluidic device of step (c), thereby releasing the rare cells from the microfluidic device.

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