Compositions, kits, and methods for isolating vesicles

• US 9,546,938 B2
• Filed: 04/04/2013
• Issued: 01/17/2017
• Est. Priority Date: 09/28/2012
• Status: Active Grant

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Kim, Ga-hee, Yoo, Chang-eun, Yong, Ye-ryoung, Lee, Myo-yong
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US13/857,087
• Publication Number:
  US 20140093880A1
• Time in Patent Office: 1,384 Days
• Field of Search: 435/6.1
• US Class Current: 1/1
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: G01N 1/405 by adsorption or absorption
• CPC Class Codes: G01N 33/5076 involving cell organelles, e.g. Golgi complex, endoplasmic reticulum
• CPC Class Codes: G01N 33/57492 involving compounds localized on the membrane of tumor or cancer cells

Abstract

A composition, a kit, and a method of isolating a vesicle from a sample using a compound comprising zwitterion moieties, which may be used to analyze vesicles, and proteins, glycoprotein, lipids, or nucleic acids thereof.

Claims

1. A method of isolating a vesicle from a sample, the method comprising:

incubating a compound comprising zwitterionic moieties with a sample comprising a vesicle to form a reaction mixture wherein the compound binds to the vesicle; and

isolating the vesicle from the reaction mixture,

wherein the sample is a body fluid sample or cell culture sample from which a cell or a cell residue has been removed.

2. The method of claim 1, wherein the sample is urine, mucus, saliva, tears, blood plasma, serum, sputum, spinal fluid, hydrothorax, nipple aspirate, lymph fluid, airway fluid, intestinal fluid, genitourinary fluid, breast milk, fluid in lymphatic system, semen, cerebrospinal fluid, fluid in organ system, ascites, cystic neoplasm fluid, amniotic fluid, or a combination thereof.

3. The method of claim 1, wherein the compound comprising zwitterionic moieties is fixed on a solid support.

4. The method of claim 1, further comprising detecting the isolated vesicle.

5. The method of claim 1, wherein the zwitterionic moieties have the following formula:

6. The method of claim 1, wherein the zwitterionic moieties are sulfobetaine (SB), carboxybetaine, phosphorylcholine, or a combination thereof.

7. The method of claim 1, wherein the compound comprising zwitterionic moieties is a polymer comprising zwitterionic moieties.

8. The method of claim 3, wherein the solid support is a magnetic bead, a silica bead, a polystyrene plate, a polystyrene bead, a glass bead, a cellulose bead, or a combination thereof.

9. The method of claim 1, wherein the vesicle is a liposome or a microvesicle.

10. The method of claim 5, wherein A₁ is ammonium, sulfonium, or phosphonium, and A₂ is carboxylic acid, phosphoric acid, or phosphonic acid;

or A₁ is carboxylic acid, phosphoric acid, or phosphonic acid, and A₂ is ammonium, sulfonium, or phosphonium.

11. The method of claim 5, wherein R₁ is a C₁-C₈ aliphatic hydrocarbon;

R₂ is a carboxyl group;

n is from about 1 to about 10; and

m is from about 1 to about 10.

12. The method of claim 10, wherein R₁ is a C₁-C₈ aliphatic hydrocarbon;

R₂ is a carboxyl group;

n is from about 1 to about 10; and

m is from about 1 to about 10.

13. The method of claim 11, wherein R₁ is a C_1-8 alkane.

14. The method of claim 12, wherein R₁ is a C_1-8 alkane, a C_1-8 alkene, or a C_1-8 alkyne.

15. The method of claim 1, wherein the vesicle comprises a nucleic acid.

16. The method of claim 1, wherein the vesicle comprises miRNA.

17. The method of claim 15, wherein the method further comprises analyzing the nucleic acid.