Mobile hemolysis detection in whole blood samples

• US 9,547,899 B1
• Filed: 10/02/2015
• Issued: 01/17/2017
• Est. Priority Date: 10/07/2014
• Status: Active Grant

• Current Assignee: University of South Florida (State University System of Florida)
• Original Assignee: University of South Florida (State University System of Florida)
• Inventors: Pyayt, Anna
• Primary Examiner(s): AZARIAN, SEYED H
• Application Number:
  US14/873,978
• Time in Patent Office: 473 Days
• Field of Search: 382/100, 382/103, 382106-107, 382128-134, 382/140, 382162-168, 382/173, 382/181, 382/190, 382/199, 382/209, 382/219, 382/224, 382/232, 382/254, 382/274, 382/276, 382/286, 382/291, 382/305, 382/312, 506/9, 600/322, 494/10, 422/82.05, 436/169
• US Class Current: 1/1
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: G06V 20/693 Acquisition

Abstract

A system for hemolysis detection, where the system generally includes—individually or in combination—a containment apparatus for holding the blood sample and a software application for analyzing the blood sample. The containment apparatus houses the sample therein, and blocks out any light from penetrating the apparatus and reflecting off of the sample. In conjunction, the software application uses camera color for processing the color of the sample to determine the hemoglobin level in plasma based on the color.

Claims

1. A system for measuring hemoglobin concentration in a whole blood sample within a short period of time after drawing said whole blood sample from a patient or subject, comprising:

a containment apparatus having a substantially hollow interior defined by a plurality of sidewalls that are light impenetrable,

said plurality of sidewalls including

left and right sidewalls,

a bottom sidewall,

a top sidewall that is removable or openable to provide access into said substantially hollow interior of said containment apparatus from a top side of said containment apparatus,

a front sidewall, and

a rear sidewall or frame that is structured to receive an electronic mobile device having a camera function;

a cuvette stand disposed on said front sidewall within said interior of said containment apparatus, said cuvette stand configured to receive and hold a cuvette containing said whole blood sample in a position directly opposite from a camera lens of said mobile device;

a flash dispersing flange positioned along said rear sidewall corresponding to a flash of said camera function of said mobile device in order to scatter any light from said flash,

wherein said mobile device includes a non-transitory computer medium that contains computer-executable instructions which when executed by a processor causes the processor causes the processor to perform the step of

receiving an image of said whole blood sample from said camera function of said mobile device,

detecting a color of a separated plasma component within said whole blood sample, and

automatically determining a hemoglobin concentration and a hemolysis level in said whole blood sample based on said detected color of said plasma component,

wherein when said top sidewall is closed and said mobile device is inserted into said rear sidewall, external light cannot penetrate into said substantially hollow interior of said containment apparatus.

2. A system as in claim 1, further comprising:

said determination of said hemoglobin concentration and said hemolysis level achieved by said computer medium comparing said detected color to a set of predetermined red/yellow calibration values built into said computer medium.

3. A system as in claim 1, further comprising:

said mobile device further including a display function, and

said computer medium further including instructions for displaying results of said determination of said hemoglobin concentration and said hemolysis level on said display function of said mobile device.

4. A system as in claim 1, further comprising:

said rear sidewall or frame being a full sidewall;

a slot formed along an outer surface of said full sidewall configured to receive said mobile device;

a camera aperture formed through said full sidewall in alignment with said lens of said mobile device when said mobile device is inserted into said slot.

5. A system as in claim 1, further comprising:

said rear sidewall or frame being a notched frame configured to receive said mobile device, such that said mobile device forms a part of said rear sidewall when inserted into said notched frame.

6. A system as in claim 1, further comprising:

a perforated section positioned along said rear sidewall corresponding to a flash of said camera function of said mobile device in order to scatter any light from said flash.

7. A system as in claim 1, further comprising:

said cuvette stand including a cuvette stand frame and a cuvette stand aperture, said cuvette stand aperture configured to receive said cuvette and said cuvette stand frame configured to hold said cuvette in position for said image to be captured by said camera function of said mobile device.

8. A system as in claim 1, further comprising:

said front sidewall having a light-colored inner surface to provide a background against which said image of said whole blood sample can be captured.

9. A method of measuring hemoglobin concentration in a whole blood sample within a short period of time after drawing said whole blood sample from a patient or subject, comprising:

providing a containment apparatus having a substantially hollow interior configured to enclose said whole blood sample, said containment apparatus having at least one (1) opening through which a mobile device can capture an image of said whole blood sample,

said substantially hollow interior defined by a plurality of sidewalls that are light impenetrable, said plurality of sidewalls including

left and right sidewalls,

a bottom sidewall,

a top sidewall that is removable or openable to provide access into said substantially hollow interior of said containment apparatus from a top side of said containment apparatus,

a front sidewall, and

a rear sidewall or frame that is structured to receive said mobile device having a camera function,

positioning a flash dispersing flange along said rear sidewall corresponding to a flash of said camera function of said mobile device in order to scatter any light from said flash,

providing on said mobile device a non-transitory computer medium that contains computer-executable instructions executed by a processor;

positioning a lens of said mobile device through said opening in said containment apparatus, such that external light cannot penetrate into said substantially hollow interior of said containment apparatus; and

capturing said image of said whole blood sample,

said processor performing the steps of

receiving said image of said whole blood sample from said mobile device,

detecting a color of a separated plasma component within said whole blood sample, and

automatically determining hemoglobin concentration and hemolysis level in said whole blood sample based on said detected color of said plasma component.

10. A method as in claim 9, further comprising:

inputting a set of predetermined red/yellow calibration values into said computer mediums, wherein said determination of said hemoglobin concentration and said hemolysis level is achieved by said computer medium comparing said detected color to said set of predetermined red/yellow calibration values.

11. A method as in claim 9, further comprising:

said mobile device further including a display function, and

said computer medium further including instructions for displaying results of said determination of said hemoglobin concentration and said hemolysis level on said display function of said mobile device.

12. A method as in claim 9, wherein:

a said rear sidewall is structured to receive said mobile device within a slot positioned along said rear sidewall; and

the step of positioning said lens of said mobile device through said opening in said containment apparatus is performed by positioning said lens of said mobile device through a camera aperture formed in said rear sidewall when said mobile device is inserted into said slot.

13. A method as in claim 12, further comprising:

positioning a flash of said mobile device through a perforated section positioned along said rear sidewall in order to scatter any light from said flash during image capture.

14. A method as in claim 9, wherein:

a said rear sidewall is formed of a notched frame configured to receive said mobile device, such that said mobile device forms a part of said rear sidewall when inserted into said notched flame.

15. A method as in claim 9, further comprising:

Providing a cuvette stand disposed on said front sidewall within said interior of said containment apparatus, said cuvette stand configured to receive and hold a cuvette containing said whole blood sample in a position directly opposite from a camera lens of said mobile device;

said cuvette stand including a cuvette stand frame and a cuvette stand aperture, said cuvette stand aperture configured to receive said cuvette and said cuvette stand frame configured to hold said cuvette in position for said image to be captured by said mobile device.

16. A method as in claim 9, further comprising:

positioning an open front side of said containment apparatus against a flat, light-colored background to provide a background against which said image of said whole blood sample can be captured.