Reactor plate and reaction processing method

• US 9,550,184 B2
• Filed: 11/19/2007
• Issued: 01/24/2017
• Est. Priority Date: 02/05/2007
• Status: Active Grant

• Current Assignee: Shimadzu Corporation
• Original Assignee: Shimadzu Corporation
• Inventors: Hanafusa, Nobuhiro, Ogata, Koretsugu, Nishimoto, Takahiro, Kanai, Masaki
• Primary Examiner(s): Dauner, Joseph G
• Application Number:
  US12/525,947
• Publication Number:
  US 20110003286A1
• Time in Patent Office: 3,354 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0684 Venting, avoiding backpressure, avoid gas bubbles
• CPC Class Codes: B01L 2200/141 Preventing contamination, tampering
• CPC Class Codes: B01L 2300/046 Function or devices integrated in the closure
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0622 distribution valves, valves having multiple inlets and/or outlets, e.g. metering valves, multi-way valves
• CPC Class Codes: B01L 2400/0644 rotary valves
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/50853 with covers or lids
• CPC Class Codes: G01N 35/1097 characterised by the valves valves in general F16K

Abstract

Disclosed herein is a reactor plate which prevents the entry of foreign matter from the outside and the pollution of a surrounding environment. A reactor plate (1) includes a reaction well (5), a reaction well channel connected to the reaction well (5), and reaction well air vent channels (19) and (21) connected to the reaction well (5). The reaction well channel has a main channel (13), a metering channel (15) branched off the main channel (13), and an injection channel (17) of which one end is connected to the metering channel (15) and the other end is connected to the reaction well (5). The main channel (13) and the reaction well air vent channel (21) can be hermetically sealed. The injection channel (17) is formed narrower than the metering channel (15) not so as to allow the passage of a liquid at a liquid introduction pressure applied to introduce the liquid into the main channel (13) and the metering channel (15) and at a purge pressure applied to purge the liquid from the main channel (13) but so as to allow the passage of the liquid at a pressure higher than the liquid introduction pressure and the purge pressure.

Claims

1. A reactor plate comprising:

a reaction well;

a reaction well channel connected to the reaction well,

the reaction well channel being constituted from a groove formed in the contact surface between two members bonded together or from the groove and a through hole formed in the member,

the reaction well channel including a main channel, a metering channel branched off the main channel and having a predetermined capacity, and an injection channel of which one end is directly connected to the metering channel and the other end is connected to the reaction well, the injection channel being formed narrower than the metering channel not so as to allow the passage of a liquid at a liquid introduction pressure applied to introduce the liquid into the main channel and the metering channel and at a purge pressure applied to purge the liquid from the main channel but so as to allow the passage of the liquid at a pressure higher than the liquid introduction pressure and the purge pressure;

a reaction well air vent channel directly connected to the reaction well and formed separately from the reaction well channel;

a variable member having an internal capacity being passively variable by expanding and contracting, the variable member being provided in the reactor plate; and

a switching valve being configured to connect the main channel and the reaction well air vent channel to the variable member so as to allow moving a gas in the main channel and the reaction well air vent channel to the variable member in a closed system;

thereby the main channel and the reaction well air vent channel are kept hermetically sealed,

wherein the main channel has an elongated structure defining a flow path extending in the direction of elongation and wherein the metering channel branches from a side of the flow path.

2. The reactor plate according to claim 1, wherein the contact angle of the injection channel with a water drop is 90°

or higher, and the area of an interface between the injection channel and the metering channel is in a range of 1 to 10,000,000 μ

m².

3. The reactor plate according to claim 1, further comprising a plurality of reaction wells, wherein a metering channel and an injection channel are provided for each of the reaction wells, and each of the metering channels is connected to the main channel.

4. The reactor plate according to claim 1, further comprising a projecting portion which projects from a top inner surface of the reaction well and has a proximal end and a distal end narrower than the proximal end, wherein the other end of the injection channel is located at the distal end of the projecting portion.

5. The reactor plate according to claim 1, further comprising a sealed well other than the reaction well.

6. The reactor plate according to claim 5, wherein the sealed well is a sample well for containing a sample liquid.

7. The reactor plate according to claim 6, wherein the sample well is sealed with an elastic member which allows a dispensing device having a sharp tip to pass through to form a through hole and also allows the through hole to be closed by pulling out the dispensing device due to its elasticity.

8. The reactor plate according to claim 6, further comprising one or more reagent wells, each of which is a sealed well, other than the sample well, wherein the reagent well is sealed with a film, or has an openable and closable cap.

9. The reactor plate according to claim 6, further comprising a gene amplification well which is a sealed well, other than the sample well and is used for carrying out gene amplification reaction.

10. The reactor plate according to claim 5, further comprising a sealed well channel connected to the sealed well, and a syringe for sending a liquid,

wherein the switching valve is also configured to connect the syringe to the main channel or the sealed well channel.

11. The reactor plate according to claim 10, wherein the switching valve is a rotary valve.

12. The reactor plate according to claim 11, wherein the rotary valve has a port to be connected to the syringe at the center of rotation and the syringe is placed on the rotary valve.

13. The reactor plate according to claim 1, wherein the reaction well is made of an optically-transparent material so that optical measurement can be carried out from the bottom of the reaction well or from above the reaction well.

14. The reactor plate according to claim 1, wherein a liquid to be injected into the reaction well contains a gene, and the reaction well contains a probe which reacts with the gene.

15. The reactor plate according to claim 1, wherein the variable member is a bellows.

16. A method for processing a reaction by using the reactor plate according to claim 1, the method comprising:

filling the main channel and the metering channel with a liquid at the liquid introduction pressure;

purging the liquid from the main channel by flowing a gas through the main channel while allowing the liquid to remain in the metering channel; and

injecting the liquid contained in the metering channel into the reaction well through the injection channel by creating a positive pressure higher than the liquid introduction pressure in the main channel, or by creating a negative pressure in the reaction well, or by creating a positive pressure higher than the liquid introduction pressure in the main channel and creating a negative pressure in the reaction well.