Vessel and process for production thereof

• US 9,550,600 B2
• Filed: 08/02/2011
• Issued: 01/24/2017
• Est. Priority Date: 08/06/2010
• Status: Active Grant

• Current Assignee: Rapid Diagnostics ML Limited
• Original Assignee: Enigma Diagnostics Limited
• Inventors: Whitaker, David, Neale, Kevin, Squirrell, David
• Primary Examiner(s): Pickett, J. Gregory
• Assistant Examiner(s): ELOSHWAY, NIKI MARINA
• Application Number:
  US13/814,545
• Publication Number:
  US 20140008376A1
• Time in Patent Office: 2,002 Days
• Field of Search: 220/675, 220/651, 220/669, 220/671
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0858 Side walls
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: B01L 3/5082 Test tubes per se
• CPC Class Codes: B01L 3/52 Containers specially adapted for storing or dispensing a reagent B01L3/02 takes precedence; containers for medical or pharmaceutical purposes A61J1/00; containers in general B65D; storing or dispensing test elements G01N33/4875; automated reagent dispensing G01N35/1002
• CPC Class Codes: B29D 22/003 Containers for packaging, storing or transporting, e.g. bottles, jars, cans, barrels, tanks
• CPC Class Codes: B65D 1/40 Details of walls other container details B65D23/00, B65D25/00

Abstract

A vessel of a malleable or moldable material having one or more side walls and a closed base integral with said one or more side walls, an opening in the top of the vessel, and further comprising two or more projections extending into the interior of the vessel generally laterally from said side walls, wherein said projections are also integral with said side wall. Vessels of this type are produced by a process in which ridges are deformed into projections within a vessel blank, and this process forms a further aspect of the invention. They are particularly useful for holding solid masses such as small beads or cakes in position in the vessel.

Claims

1. A vessel for carrying out chemical or biochemical reactions, said vessel comprising:

a malleable or mouldable material, the vessel having one or more side walls and a closed base integral with said one or more side walls, the one or more side walls defining an upper portion and a lower portion of relatively smaller cross sectional area having a tapering profile, the upper portion having an opening;

a reagent chamber in the lower portion;

two or more projections located in an area of a junction between the upper and lower portions, said two or more projections extending into an interior of the vessel, generally laterally from said one or more side walls, wherein said projections are also integral with said side wall; and

a freeze dried reagent retained within a region located below the projections, the projections forming a restraint that prevents the freeze dried reagent being displaced within the vessel or exiting through the opening in the upper portion.

2. The vessel of claim 1 wherein the projections are in the form of flanges, teeth or tines.

3. The vessel of claim 1 wherein said two or more projections are arranged such that they project generally inwardly and upwardly within the vessel.

4. The vessel of claim 1 wherein the projections are spaced around said one or more side walls of the vessel at a similar level therein.

5. The vessel of claim 1 wherein the projections are resilient.

6. A method for loading the vessel of claim 5, the method comprising forcing a solid freeze dried reagent past resilient projections within the vessel.

7. The vessel of claim 1 wherein the vessel is of generally circular cross-section.

8. The vessel of claim 1 wherein the projections are spaced downwards from the opening of the vessel.

9. The vessel of claim 1 wherein the vessel comprises an upper portion having a relatively expanded cross sectional area, which accommodates the opening.

10. The vessel of claim 1 wherein the malleable or mouldable material is a plastics material.

11. The vessel of claim 10 wherein the plastics material is an electrically conducting polymeric material.

12. A method of preparing the vessel of claim 1, said method comprising:

(i) forming from a malleable or mouldable material, a vessel blank comprising a bottom and integral side walls and having one or more vertically arranged ridges arranged on an inner surface of said side walls; and

subsequently (ii) introducing a deforming tool into said vessel blank which tool is able to deform the top of said one or more ridges radially inwards so as to produce said one or more inwardly directed projections therefrom, (iii) withdrawing the deforming tool from the thus formed vessel, and (iv) loading the region of the vessel below the projections with the freeze dried reagent.

13. The method of claim 12 wherein the vessel blank is retained in an external mould during step (ii).

14. The method of claim 12, wherein the step of loading the region of the vessel below the projections with the freeze dried reagent is selected from (i) forcing the freeze dried reagent past resilient projections within the vessel, and (ii) adding a solution of reagents to the said region and subjecting said solution to a lyophilisation step to produce the freeze dried reagent therefrom.

15. The method of claim 12, wherein the step of loading the region of the vessel below the projections with the freeze dried reagent comprises adding a solution of reagents to said region and subjecting said solution to a lyophilisation step to produce a solidified cake therefrom.

16. The method of claim 12, wherein the inwardly directed projections comprise resilient projections and wherein the step of loading the region of the vessel below the projections with the freeze dried reagent comprises forcing a mass of solid material past said resilient projections.

17. A method for loading the vessel of claim 1, the method comprising adding a solution of reagents to said vessel so that it is accommodated below or in the region of the projections and subjecting said solution to a lyophilisation step to produce a solidified cake therefrom.