Nucleic acid sequence analysis
First Claim
1. A method of sequencing a single template molecule, the method comprising:
- localizing the single template molecule to a reaction region through association with a template localizing moiety attached to the reaction region, wherein the template localizing moiety comprises a protein that translocates along the single template molecule and does not synthesize a polynucleotide complementary to the single template molecule while retaining the single template molecule at the reaction region; and
sequencing a polynucleotide region of the localized single template molecule to obtain a first polynucleotide sequence read of the polynucleotide region, wherein the polynucleotide sequence read comprises redundant sequence information for the polynucleotide region, thereby sequencing the single template molecule.
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Abstract
Provided are methods for sequencing a nucleic acid with a sequencing enzyme, e.g., a polymerase or exonuclease. The sequencing enzyme can optionally be exchanged with a second sequencing enzyme, which continues the sequencing of the nucleic acid. In certain embodiments, a template is fixed to a surface through a template localizing moiety. The template localizing moiety can optionally anneal with the nucleic acid and/or associate with the sequencing enzyme. Also provided are compositions comprising a nucleic acid and a first sequencing enzyme, which can sequence the nucleic acid and optionally exchange with a second sequencing enzyme present in the composition. Compositions in which a template localizing moiety is immobilized on a surface are provided. Also provided are methods for using data from analytical reactions wherein two different enzymes are employed, e.g., at a same or different reaction regions.
43 Citations
21 Claims
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1. A method of sequencing a single template molecule, the method comprising:
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localizing the single template molecule to a reaction region through association with a template localizing moiety attached to the reaction region, wherein the template localizing moiety comprises a protein that translocates along the single template molecule and does not synthesize a polynucleotide complementary to the single template molecule while retaining the single template molecule at the reaction region; and sequencing a polynucleotide region of the localized single template molecule to obtain a first polynucleotide sequence read of the polynucleotide region, wherein the polynucleotide sequence read comprises redundant sequence information for the polynucleotide region, thereby sequencing the single template molecule. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A method of sequencing a single nucleic acid molecule, the method comprising:
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localizing the single nucleic acid molecule to a reaction region through association with a template localizing moiety attached to the reaction region, wherein the template localizing moiety comprises a protein selected from the group consisting of;
a helicase, a sliding clamp, a DNA repair enzyme, and a polymerase mutant, fragment, or subunit thereof that does not synthesize a polynucleotide complementary to the single template molecule; andsequencing a polynucleotide region of the localized single template nucleic acid molecule using a method selected from the group consisting of exonuclease sequencing, nanopore-based sequencing, and sequencing by synthesis to obtain a polynucleotide sequence read of the polynucleotide region, thereby sequencing the single nucleic acid molecule. - View Dependent Claims (12, 13, 14, 15)
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16. A method of generating redundant sequence information for a polynucleotide region from multiple template nucleic acid molecules, the method comprising:
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providing multiple template nucleic acid molecules, each of which comprises the same polynucleotide region; localizing each of the template nucleic acid molecules to a different reaction region through association with a template localizing moiety attached to each reaction region, wherein each template localizing moiety encircles each template nucleic acid molecule such that a strand of said template nucleic acid molecule can move through the template localizing moiety, and further wherein the template localizing moiety does not synthesize a polynucleotide complementary to the single template molecule; sequencing the polynucleotide region in each of the template nucleic acid molecules using a method selected from the group consisting of;
exonuclease sequencing, nanopore-based sequencing, and sequencing by synthesis to obtain multiple polynucleotide sequence reads of the polynucleotide region, wherein the polynucleotide sequence reads comprises redundant sequence information for the polynucleotide region, thereby generating redundant sequence information from the template nucleic acid molecules. - View Dependent Claims (17, 18, 19, 20, 21)
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Specification