System and method for creating a flow cytometer network
First Claim
1. A system for creating a flow cytometer network, comprising:
- a first and second flow cytometers, each flow cytometer respectively including;
an interrogation zone through which a flow cytometer sample passes; and
a fixed gain detection system configured to use a fixed gain setting for collecting for the sample a sample data set of photonic inputs from the interrogation zone;
a flow cytometer data center that stores and manages sample-related data from the first and second flow cytometers, the flow cytometer data center comprising an analysis module configured to perform data analysis by directly comparing the sample data sets from the first and the second flow cytometers without compensating for differences between the fixed gain settings of the first and the second flow cytometers, and without compensating for differences in calibration settings of the first and the second flow cytometers; and
a network communication module communicatively coupled to at least one of the first and the second flow cytometers and the flow cytometer data center, wherein the network communication module communicates sample-related data between the at least one of the first and the second flow cytometers and the flow cytometer data center.
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Abstract
A system for creating a flow cytometer network includes: a flow cytometer with an interrogation zone and a fixed gain detection system that collects sample data from the interrogation zone; a flow cytometer data center that stores and manages sample-related data from the flow cytometer; and a network communication module that communicates sample-related data between the flow cytometer and the data center. The system may include a second flow cytometer and a second network communication module, where the first and second flow cytometers are calibrated to have substantially identical fixed gain settings. A method for creating a flow cytometer network includes: calibrating first and second flow cytometers with a calibration solution; collecting sample data with a fixed gain detection system; uploading data to a flow cytometer data center; retrieving data from the data center; and performing data analysis on the retrieved data.
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Citations
25 Claims
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1. A system for creating a flow cytometer network, comprising:
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a first and second flow cytometers, each flow cytometer respectively including; an interrogation zone through which a flow cytometer sample passes; and a fixed gain detection system configured to use a fixed gain setting for collecting for the sample a sample data set of photonic inputs from the interrogation zone; a flow cytometer data center that stores and manages sample-related data from the first and second flow cytometers, the flow cytometer data center comprising an analysis module configured to perform data analysis by directly comparing the sample data sets from the first and the second flow cytometers without compensating for differences between the fixed gain settings of the first and the second flow cytometers, and without compensating for differences in calibration settings of the first and the second flow cytometers; and a network communication module communicatively coupled to at least one of the first and the second flow cytometers and the flow cytometer data center, wherein the network communication module communicates sample-related data between the at least one of the first and the second flow cytometers and the flow cytometer data center. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for creating a flow cytometer network, comprising:
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calibrating a first flow cytometer with a calibration solution; calibrating a second flow cytometer with the calibration solution; collecting, at the first flow cytometer a first sample data set for a first sample using a first fixed gain detection system with a first fixed gain setting; collecting, at the second flow cytometer a second sample data set for a second sample using a second fixed gain detection system with a second fixed gain setting; uploading the first and the second sample data sets to a flow cytometer data center; retrieving flow cytometry data from the flow cytometer data center; and performing, at the flow cytometer data center, data analysis by directly comparing the first and the second sample data sets without compensating for differences between the first fixed gain setting and the second fixed gain setting, and without compensating for differences in calibration settings of the first and the second flow cytometers. - View Dependent Claims (17, 18, 19, 20, 21, 22)
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23. A method for creating a flow cytometer network, comprising:
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calibrating a first flow cytometer with a calibration solution; calibrating a second flow cytometer with the calibration solution; collecting, at the first flow cytometer a first sample data set for a first sample using a first fixed gain detection system with a first fixed gain setting, wherein collecting the first sample data set includes collecting photonic inputs from an interrogation zone of the first flow cytometer across a wide dynamic range that includes at least a 1;
100,000 ratio between the faintest objects and the brightest objects;collecting, at the second flow cytometer a second sample data set for a second sample using a second fixed gain detection system with a second fixed gain setting, wherein collecting the second sample data set includes collecting photonic inputs from an interrogation zone of the second flow cytometer across a wide dynamic range; uploading the first and the second sample data sets to a flow cytometer data center; retrieving flow cytometry data from the flow cytometer data center; and performing, at the flow cytometer data center, data analysis by directly comparing the first and the second sample data sets without compensating for differences between the first fixed gain setting and the second fixed gain setting. - View Dependent Claims (24)
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25. A method for creating a flow cytometer network, comprising:
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calibrating a first flow cytometer with a calibration solution; calibrating a second flow cytometer with the calibration solution; collecting, at the first flow cytometer a first sample data set for a first sample using a first fixed gain detection system with a first fixed gain setting, wherein collecting the first sample data set includes collecting photonic inputs from an interrogation zone across a wide dynamic range that includes a 1;
100,000 ratio between the faintest objects and the brightest objects;collecting, at the second flow cytometer a second sample data set for a second sample using a second fixed gain detection system with a second fixed gain setting; uploading the first and the second sample data sets to a flow cytometer data center; retrieving flow cytometry data from the flow cytometer data center; and performing, at the flow cytometer data center, data analysis by directly comparing the first and the second sample data sets without compensating for differences between the first fixed gain setting and the second fixed gain setting, and without compensating for differences in calibration settings of the first and the second flow cytometers.
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Specification