Flow cytometry apparatus and methods

• US 9,551,645 B2
• Filed: 07/07/2015
• Issued: 01/24/2017
• Est. Priority Date: 07/10/2014
• Status: Active Grant

• Current Assignee: Kinetic River Corp.
• Original Assignee: Kinetic River Corp.
• Inventors: Vacca, Giacomo
• Primary Examiner(s): Punnoose, Roy M
• Application Number:
  US14/793,626
• Publication Number:
  US 20160011096A1
• Time in Patent Office: 567 Days
• Field of Search: 356/342
• US Class Current: 1/1
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: G01N 15/1436 the optical arrangement forming an integrated apparatus with the sample container, e.g. a flow cell
• CPC Class Codes: G01N 15/1459 the analysis being performed on a sample stream
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1415 Control of particle position

Abstract

A particle analyzer, comprising a source of a substantially nondiffracting light beam; a flow path configured to produce in a flowcell a ribbon-like core stream having a cross-sectional aspect ratio of at least 4 and a largest cross-sectional dimension of at least 50 micrometers; the flowcell being configured to expose a segment of the core stream to the light beam; a detector configured to receive a signal resulting from an interaction of a particle in the core stream with the light beam; a first sorting actuator connected with the flowcell, downstream of the exposed segment of core stream; a plurality of sorting channels in fluid connection with the flow path and downstream of the first actuator; the actuator having multiple actuation states, each state configured to direct at least one part of the core stream to a corresponding channel; a second sorting actuator connected with the flowcell, opposite the first actuator, and operable in coordination with the first actuator.

Claims

1. A particle analyzer, comprising:

a source of a non-Gaussian, substantially nondiffracting light beam;

a flow path configured to produce a ribbon-like core stream in a flowcell, said core stream having a cross-sectional aspect ratio of at least 4 and a largest cross-sectional dimension of at least 50 micrometers;

said flowcell being configured to expose a segment of said core stream to said light beam; and

a detector configured to detect a signal from said core stream, the signal resulting from an interaction of a particle in said core stream with said light beam.

2. The particle analyzer of claim 1, further comprising:

a first sorting actuator connected with said flowcell and downstream of said segment of said core stream exposed to said light beam; and

a plurality of sorting channels in fluid connection with said flow path and downstream of said first sorting actuator;

said first sorting actuator having multiple actuation states, each actuation state configured to direct at least one part of said core stream to a corresponding sorting channel of said plurality of sorting channels.

3. The particle analyzer of claim 2, further comprising a second sorting actuator, said second sorting actuator being connected with said flowcell and opposite said first sorting actuator, said second sorting actuator being operable in coordination with said first sorting actuator.

4. The particle analyzer of claim 1, wherein said light beam is aligned with the largest cross-sectional dimension of said core stream under an angle of no more than 45 degrees.

5. The particle analyzer of claim 1, wherein said light beam is aligned substantially along the largest cross-sectional dimension of said core stream.

6. A particle analyzer, comprising:

a source of a non-Gaussian, substantially nondiffracting light beam;

a flow path configured to produce a ribbon-like core stream in a flowcell, said core stream having a cross-sectional aspect ratio of at least 4 and a largest cross-sectional dimension of at least 50 micrometers;

said flowcell being configured to expose a segment of said core stream to said light beam; and

a plurality of detectors, whereby individual detectors in said plurality of detectors are configured to receive a signal from a corresponding portion of said core stream, said signal resulting from an interaction of a particle in said corresponding portion of said core stream with said light beam.

7. The particle analyzer of claim 6, further comprising:

a first plurality of sorting actuators connected with said flowcell, said first plurality of sorting actuators being positioned downstream of said segment of said core stream exposed to said light beam;

said first plurality of sorting actuators being in substantial alignment with the largest cross-sectional dimension of said core stream; and

a plurality of sorting channels in fluid connection with said flow path and downstream of said first plurality of sorting actuators;

the sorting actuators of said first plurality of sorting actuators having multiple actuation states, each actuation state configured to direct at least one part of said core stream to a corresponding sorting channel of said plurality of sorting channels.

8. The particle analyzer of claim 7, further comprising a second plurality of sorting actuators, said second plurality of sorting actuators being connected with said flowcell and opposite said first plurality of sorting actuators, actuators in said second plurality of sorting actuators being operable in coordination with actuators in said first plurality of sorting actuators.

9. The particle analyzer of claim 6, wherein said light beam is aligned with the largest cross-sectional dimension of said core stream under an angle of no more than 45 degrees.

10. The particle analyzer of claim 6, wherein said light beam is aligned substantially along the largest cross-sectional dimension of said core stream.

11. A particle analyzer, comprising:

a source of a light beam;

a flow path configured to produce a ribbon-like core stream in a flowcell, said core stream having a cross-sectional aspect ratio of at least 4 and a largest cross-sectional dimension of at least 50 micrometers;

said flowcell being configured to expose a segment of said core stream to said light beam; and

a plurality of detectors, whereby individual detectors in said plurality of detectors are configured to receive a signal from a corresponding portion of said core stream, said signal resulting from an interaction of a particle in said corresponding portion of said core stream with said light beam.

12. The particle analyzer of claim 11, further comprising:

a first plurality of sorting actuators connected with said flowcell, said plurality of sorting actuators being positioned downstream of said segment of said core stream exposed to said light beam;

said plurality of sorting actuators being in substantial alignment with the largest cross-sectional dimension of said core stream; and

a plurality of sorting channels in fluid connection with said flow path and downstream of said first plurality of sorting actuators;

the sorting actuators of said first plurality of sorting actuators having multiple actuation states, each actuation state configured to direct at least one part of said core stream to a corresponding sorting channel of said plurality of sorting channels.

13. The particle analyzer of claim 12, further comprising a second plurality of sorting actuators, said second plurality of sorting actuators being connected with said flowcell and opposite said first plurality of sorting actuators, actuators in said second plurality of sorting actuators being operable in coordination with actuators in said first plurality of sorting actuators.

14. The particle analyzer of claim 11, wherein said light beam is aligned with the largest cross-sectional dimension of said core stream under an angle of no more than 45 degrees.

15. The particle analyzer of claim 11, wherein said light beam is aligned substantially along the largest cross-sectional dimension of said core stream.