Target detection
First Claim
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1. A method for detecting a pathogen and confirming proper functioning of an assay, the method comprisingobtaining a sample suspected of containing a pathogen;
- introducing a detectable marker into the sample;
introducing magnetic particles into the sample;
incubating the sample to allow for coupling of the detectable marker and the pathogen to the magnetic particles, wherein the detectable marker and the pathogen couple to separate binding moieties;
conducting an assay using the magnetic particles to isolate and determine presence of both the detectable marker from the sample and the pathogen, wherein the presence of the pathogen is determined separate from the presence of the detectable marker, and wherein determination of the presence of the detectable marker confirms proper functioning of the assay.
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Abstract
The invention generally relates to methods for indicating whether an assay for isolating targets is properly isolating and detecting targets. Methods of the invention involve obtaining a sample suspected of a containing target, introducing a detectable marker into the sample, conducting an assay using magnet particles to isolate the detectable marker and the target if it is present in the sample, determining the presence or absence of the target; and confirming that the assay functioned properly by determining presence or absence of the detectable marker.
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Citations
13 Claims
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1. A method for detecting a pathogen and confirming proper functioning of an assay, the method comprising
obtaining a sample suspected of containing a pathogen; -
introducing a detectable marker into the sample; introducing magnetic particles into the sample; incubating the sample to allow for coupling of the detectable marker and the pathogen to the magnetic particles, wherein the detectable marker and the pathogen couple to separate binding moieties; conducting an assay using the magnetic particles to isolate and determine presence of both the detectable marker from the sample and the pathogen, wherein the presence of the pathogen is determined separate from the presence of the detectable marker, and wherein determination of the presence of the detectable marker confirms proper functioning of the assay. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13)
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Specification