Amplification reporter with base-pairing oligomers
First Claim
1. A method of analysis, the method comprising:
- forming at least one volume containing a reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter to affect a photoluminescence detectable from a photoluminophore of the first oligomer, wherein the first oligomer and the second oligomer are not covalently linked to one another;
amplifying a target in the at least one volume, at least in part by extending one or more primers at a temperature above the melting temperature of the reporter, wherein the step of amplifying causes cleavage of the first oligomer, and wherein the cleavage affects the photoluminescence detectable from the photoluminophore;
detecting photoluminescence from the photoluminophore in the at least one volume while the at least one volume is at a temperature below the melting temperature of the reporter; and
determining a level of the target based on the photoluminescence detected.
1 Assignment
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Accused Products
Abstract
System, including methods, apparatus, and compositions, for performing amplification assays with an amplification reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter. The reporter may have a detectable photoluminescence that is affected, such as reduced, by base-pairing of the first and second oligomers with one another. A target, such as a nucleic acid target sequence, may be amplified in at least one volume, such as a plurality of partitions, above the melting temperature, and photoluminescence of the reporter may be detected from the at least one volume below the melting temperature. A property of the target, such as a concentration of the target, may be determined based on the photoluminescence detected.
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Citations
10 Claims
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1. A method of analysis, the method comprising:
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forming at least one volume containing a reporter including a first oligomer and a second oligomer capable of base-pairing with one another below a melting temperature of the reporter to affect a photoluminescence detectable from a photoluminophore of the first oligomer, wherein the first oligomer and the second oligomer are not covalently linked to one another; amplifying a target in the at least one volume, at least in part by extending one or more primers at a temperature above the melting temperature of the reporter, wherein the step of amplifying causes cleavage of the first oligomer, and wherein the cleavage affects the photoluminescence detectable from the photoluminophore; detecting photoluminescence from the photoluminophore in the at least one volume while the at least one volume is at a temperature below the melting temperature of the reporter; and determining a level of the target based on the photoluminescence detected. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification