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Intermittent detection during analytical reactions

  • US 9,556,480 B2
  • Filed: 05/11/2015
  • Issued: 01/31/2017
  • Est. Priority Date: 03/28/2008
  • Status: Active Grant
First Claim
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1. A method of performing a nucleic acid sequencing reaction, comprising:

  • a) preparing a reaction mixture comprising components of the nucleic acid sequencing reaction including a polynucleotide to be sequenced;

    b) initiating the nucleic acid sequencing reaction in the reaction mixture to begin progression of the nucleic acid sequencing reaction, wherein the nucleic acid sequencing reaction is initiated during a non-illuminated period, and wherein the reaction mixture comprises an enzyme selected from the group consisting of;

    a polymerase, a ligase, a ribosome, a nuclease, and a kinase; and

    c) maintaining conditions that allow the nucleic acid sequencing reaction to proceed while subjecting the reaction mixture to at least one illuminated period following the non-illuminated period, wherein illumination data is collected during the at least one illuminated period, thereby performing the nucleic acid sequencing reaction.

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