Universal multidetection system for microplates

• US 9,557,217 B2
• Filed: 05/31/2012
• Issued: 01/31/2017
• Est. Priority Date: 02/13/2007
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: BTI Holdings, Inc.
• Inventors: Venditti, Dan J. Jr., Zimenkov, Oleg, Amouretti, Xavier, Kontorovich, Michael, Norris, Ben, Sears, Richard N., Many, Christopher, Anderson, Bill, Knight, Ben, Piette, James, Piette, Ross, Tobey, Joe, Ferris, Brian
• Primary Examiner(s): Stock, Jr., Gordon J
• Application Number:
  US13/485,446
• Publication Number:
  US 20120300194A1
• Time in Patent Office: 1,706 Days
• US Class Current: 1/1
• CPC Class Codes: G01J 3/0218 using optical fibers
• CPC Class Codes: G01J 3/0224 using polarising or depolarising elements
• CPC Class Codes: G01J 3/0235 using means for replacing an element by another, for replacing a filter or a grating
• CPC Class Codes: G01J 3/0264 Electrical interface; User interface
• CPC Class Codes: G01J 3/027 Control of working procedures of a spectrometer; Failure detection; Bandwidth calculation
• CPC Class Codes: G01J 3/0291 Housings; Spectrometer accessories; Spatial arrangement of elements, e.g. folded path arrangements
• CPC Class Codes: G01J 3/0294 Multi-channel spectroscopy
• CPC Class Codes: G01J 3/10 Arrangements of light sources specially adapted for spectrometry or colorimetry
• CPC Class Codes: G01J 3/42 Absorption spectrometry; Double beam spectrometry; Flicker spectrometry; Reflection spectrometry beam switching arrangements G01J3/08
• CPC Class Codes: G01J 3/4406 Fluorescence spectrometry
• CPC Class Codes: G01J 3/443 Emission spectrometry
• CPC Class Codes: G01N 2021/6463 Optics
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/6447 by visual observation
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence

Abstract

An apparatus for optically analyzing a sample may include an imaging subsystem that images the sample, one or more analyzing subsystems that analyze the sample, a temperature control subsystem that controls a temperature of the atmosphere within the apparatus, a gas control subsystem that controls a composition of the atmosphere within the apparatus, and a control module that controls the various subsystems of the apparatus.

Claims

1. A device for analyzing one or more samples, comprising:

a receptacle support configured to support a microplate comprising a microplate well configured to hold a sample;

an incubation chamber within a housing configured to incubate the sample;

an imaging subsystem disposed substantially below the receptacle support and configured to image the sample on a cell level from below the sample, the imaging subsystem comprising an objective that is movably supported to be inserted into and removed from the incubation chamber;

a non-imaging analyzing subsystem that analyzes the sample on a well level, the non-imaging analyzing subsystem configured to provide a first measurement modality to measure an absorbance of the sample, a second measurement modality to measure fluorescence of the sample, and a third measurement modality to measure chemiluminescence of the sample;

a positioning subsystem configured to position the receptacle support to one or more positions within the housing for the non-imaging analyzing subsystem to analyze the sample and the imaging subsystem to image the sample; and

the housing that forms an exterior of the device and that encloses the receptacle support, the analyzing subsystem, the positioning subsystem, the incubation chamber, and the imaging subsystem,

wherein the receptacle support, the analyzing subsystem, the positioning subsystem, the incubation chamber, and the imaging subsystem are integrated within the housing.

2. The device of claim 1, further comprising:

a gas control module configured to control a composition of atmosphere within the housing.

3. The device of claim 1, wherein the microplate comprises a plurality of microplate wells arranged in a matrix.

4. The device of claim 1, wherein the positioning subsystem is further configured to position the receptacle support based on an index that identifies positions of a plurality of wells of the microplate.

5. The device of claim 1, wherein the objective comprises a plurality of objectives mounted on a turret, the plurality of objectives mounted on the turret to be selectable.

6. The device of claim 1, wherein the imaging subsystem comprises an imaging light source and the non-imaging analyzing subsystem comprises at least one separate analyzing light source.

7. The device of claim 1, further comprising a controller configured to control at least one of atmospheric temperature within the incubation chamber and gas composition within the housing.

8. The device of claim 1, wherein (i) one of the imaging subsystem and the non-imaging analyzing subsystem and (ii) the receptacle support share a commonly controlled atmospheric composition within the housing.

9. The device of claim 1, wherein the imaging subsystem, the non-imaging analyzing subsystem, and the receptacle support share a commonly controlled atmospheric composition within the housing.

10. The device of claim 1, further comprising:

a temperature control subsystem configured to control a temperature of an atmosphere at least within the incubation chamber.

11. The device of claim 1, further comprising:

a processor configured to control operations of the non-imaging analyzing subsystem and the imaging subsystem.

12. The device of claim 1, further comprising a temperature control subsystem configured to control atmospheric temperature and a gas control subsystem configured to control atmospheric composition around the sample within the housing to provide an environment for the sample that is conducive to cell viability.

13. The device of claim 10, wherein the temperature within the housing of the device is maintained at ambient temperature by:

a plate that conducts heat from an atmosphere within the housing;

a fan outside the housing that cools the plate through forced convection.

14. The device of claim 11, further comprising:

a user interface configured to receive a user input to control the operations of the non-imaging analyzing subsystem and the imaging subsystem.

15. The device of claim 12, wherein the receptacle support, the positioning subsystem, the non-imaging analyzing subsystem, the imaging subsystem, the gas control subsystem, and the temperature control subsystem are integrated within the housing.

16. The device of claim 15, further comprising:

a processor configured to control operations of the non-imaging analyzing subsystem, the positioning subsystem, the imaging subsystem, the gas control subsystem, and the temperature control subsystem.

17. The device of claim 16, further comprising:

a user interface configured to receive a user input to control the operations of the non-imaging analyzing subsystem, the imaging subsystem, the positioning subsystem, the gas control subsystem, and the temperature control subsystem.

18. A device for analyzing one or more samples, comprising:

a receptacle support configured to support a microplate comprising a microplate well configured to hold a sample;

an incubated measurement chamber within a housing that encloses the receptacle support and configured to incubate the sample;

an imaging subsystem disposed substantially outside the incubated measurement chamber, the imaging subsystem comprising a plurality of objectives movably supported to be inserted into and removed from the incubated measurement chamber, the imaging subsystem configured to image the sample on the cell level from below the sample;

a non-imaging analyzing subsystem disposed substantially outside the incubated measurement chamber, the non-imaging analyzing subsystem configured to analyze the sample on the well level, and configured to provide a first measurement modality to measure absorbance of the sample, a second measurement modality to measure fluorescence of the sample, and a third measurement modality to measure chemiluminescence of the sample;

a common to both the imaging subsystem and the non-imaging analyzing subsystem positioning subsystem, configured to position the receptacle support within the incubated measurement chamber; and

the housing that forms an exterior of the device and that encloses the receptacle support, the incubated measurement chamber, the analyzing subsystem, the positioning subsystem and the imaging subsystem,

wherein the receptacle support, the incubated measurement chamber, the analyzing subsystem, the positioning subsystem, and the imaging subsystem are integrated within the housing.