Measuring arrangement for optically evaluating a chemical reaction quantitatively

• US 9,557,260 B2
• Filed: 12/14/2011
• Issued: 01/31/2017
• Est. Priority Date: 12/14/2010
• Status: Active Grant

• Current Assignee: GENSPEED Biotech GmbH
• Original Assignee: Greiner Bio-One Incorporated
• Inventors: Sonnleitner, Max
• Primary Examiner(s): Turk, Neil N
• Application Number:
  US13/993,799
• Publication Number:
  US 20130294973A1
• Time in Patent Office: 1,875 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 2300/022 Transponder chips
• CPC Class Codes: B01L 2300/049 Valves integrated in closure
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 2021/7783 Transmission, loss
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 21/17 Systems in which incident light is modified in accordance with the properties of the material investigated where the material investigated is optically excited causing a change in wavelength of the incident light G01N21/63
• CPC Class Codes: G01N 21/6454 using an integrated detector array
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 21/82 : producing a precipitate or turbidity

Abstract

The invention relates to a measuring arrangement for optically evaluating a chemical reaction quantitatively, comprising a sample carrier having a carrier layer and a sample layer having an analysis side and a light outlet side opposite the analysis side, and comprising a photosensitive sensor having a plurality of photodetectors on a carrying body and having a transparent surface layer arranged over the photodetectors. A plurality of test sections are arranged on the analysis side at a distance from each other in a longitudinal direction of the sample layer. The analysis side is arranged on the carrier layer in such a way that the test sections face a volume of a microfluidic system. The sample carrier is detachably arranged in an accommodating device, so that the light outlet side faces the photosensitive sensor and the test sections are arranged over the photodetectors. Furthermore, the distance between the test sections and the photodetectors is less than 700 μm.

Claims

1. A measuring arrangement for optically evaluating a chemical reaction quantitatively, comprising

a sample carrier having a transparent carrier layer and a sample layer,

a photosensitive sensor with a plurality of photodetectors on a carrier body and a transparent cover layer arranged over the photodetectors,

wherein the photosensitive sensor is arranged in a base body,

the carrier layer comprising a discharge section, a microfluidic system and a reservoir,

the discharge section being connected via the microfluidic system to the reservoir, and the microfluidic system comprising at least one channel,

the sample layer having an analysis side and opposite the analysis side a light outlet side, wherein on the analysis side spaced apart from one another in a longitudinal direction of the sample layer, a plurality of test sections are arranged, wherein each test section of the plurality of test sections comprises a reagent corresponding to an analyte in a sample material transported in the microfluidic system for producing the reaction, and each test section of the plurality of test sections is a single body which is connected to the sample layer and the sample layer is arranged with the analysis side on the carrier layer such that the test sections face a volume of the channel of the microfluidic system and

the sample carrier being arranged detachably in a mounting device of the base body, so that the light outlet side faces the photosensitive sensor and the test sections are arranged above the photodetectors,

wherein to evaluate a result of the reaction, which is directly connected to the quantitative presence of the analyte in the sample material, the photosensitive sensor is configured to capture the test section and evaluate the time-dependent behavior of a detected optical signal,

wherein the cover layer and the sample layer are disposed between the test sections and the photodetectors, a thickness of the cover layer is less than 500 μ

m, and a thickness of the sample layer is less than 200 μ

m, such that a distance between the test sections and the photodetectors is less than 700 μ

m and at least 99.5% of the electromagnetic radiation coming from a test section and reaching the photosensitive sensor is incidental on three photodetectors,

wherein the plurality of photodetectors comprises a first photodetector disposed directly underneath a first test section of the plurality of test sections, a second photodetector disposed directly underneath a second test section of the plurality of test sections, and at least three additional photodetectors disposed between the first photodetector and the second photodetector, the first test section being disposed adjacent the second test section, and

wherein a center-to-center distance between the first test section and the second test section is greater than or equal to 3-times the center-to-center distance of adjacent photodetectors.

2. The measuring arrangement as claimed in claim 1, wherein the photosensitive sensor comprises at least 32 photodetectors.

3. The measuring arrangement as claimed in claim 1, wherein the space between two adjacent photodetectors is less than 150 μ

m.

4. The measuring arrangement as claimed in claim 1, wherein the photosensitive sensor comprises at least two rows of photodetectors arranged spaced apart from one another.

5. The measuring arrangement as claimed in claim 1, wherein the at least one channel of the microfluidic system arranged above the photodetector has a length of 30-50 mm, a width of 1-4 mm and a height of 10-200 μ

m.

6. The measuring arrangement as claimed in claim 1, further comprising a sample deposited on the discharge section in the at least one channel and a pressure gradient formed with a resulting capillary force in the direction of the reservoir.

7. The measuring arrangement as claimed in claim 1, wherein the cover layer of the photodetector has a transparency maximum in a spectral range of 400-600 nm.

8. The measuring arrangement as claimed in claim 1, wherein a boundary surface of the at least one channel, which boundary surface is arranged opposite the photosensitive sensor is optically reflective and/or the carrier layer is optically reflective.

9. The measuring arrangement as claimed in claim 1, wherein the light outlet side has a light deflecting structure at least in some sections.

10. The measuring arrangement as claimed in claim 1, wherein the sample layer and/or the cover layer is in the form of a fiber-optic panel, as a tight pack of optical fibers arranged next to one another in a row.

11. The measuring arrangement as claimed in claim 1, wherein an immersion layer is applied onto the light outlet side or the cover layer.

12. The measuring arrangement as claimed in claim 1, wherein an identity or identification mark is arranged on the sample carrier.

13. The measuring arrangement as claimed in claim 1, wherein the sample layer and/or the carrier layer is configured as an optical polarizer.

14. A measuring arrangement for optically evaluating a chemical reaction quantitatively, comprising

a sample carrier having a transparent carrier layer and a sample layer,

a photosensitive sensor with a plurality of photodetectors on a carrier body and a transparent cover layer arranged over the photodetectors,

wherein the photosensitive sensor is arranged in a base body,

the carrier layer comprising a discharge section, a microfluidic system and a reservoir,

the discharge section being connected via the microfluidic system to the reservoir, and the microfluidic system comprising at least one channel,

the sample layer having an analysis side and opposite the analysis side a light outlet side, wherein on the analysis side spaced apart from one another in a longitudinal direction of the sample layer, a plurality of test sections are arranged, wherein each test section of the plurality of test sections comprises a reagent corresponding to an analyte in a sample material transported in the microfluidic system for producing the reaction, and each test section of the plurality of test sections is a single body which is connected to the sample layer and the sample layer is arranged with the analysis side on the carrier layer such that the test sections face a volume of the channel of the microfluidic system and

the sample carrier being arranged detachably in a mounting device of the base body, so that the light outlet side faces the photosensitive sensor and the test sections are arranged above the photodetectors,

wherein to evaluate a result of the reaction, which is directly connected to the quantitative presence of the analyte in the sample material, the photosensitive sensor is configured to capture the test section and evaluate the time-dependent behavior of a detected optical signal,

wherein the cover layer and the sample layer are disposed between the test sections and the photodetectors, a thickness of the cover layer is less than 500 μ

m, and a thickness of the sample layer is less than 200 μ

m, such that a distance between the test sections and the photodetectors is less than 700 μ

m,

wherein the plurality of photodetectors comprises a first photodetector disposed directly underneath a first test section of the plurality of test sections, a second photodetector disposed directly underneath a second test section of the plurality of test sections, and at least three additional photodetectors disposed between the first photodetector and the second photodetector, the first test section being disposed adjacent the second test section, and

wherein a center-to-center distance between the first test section and the second test section is greater than or equal to 3-times the center-to-center distance of adjacent photodetectors.