Use of microfluidic systems in the electrochemical detection of target analytes
First Claim
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1. A microfluidic device for the detection of a target analyte in a fluid sample, said device comprising:
- a) a solid support comprising a first plurality of electrodes configured for electrokinetic movement of at least a portion of the sample upon application of a voltage; and
b) a detection module comprising a second plurality of electrodes configured for electrochemical detection of the target analyte, each comprising a binding ligand and a self-assembled monolayer;
wherein the electrokinetic movement is electroosmotic movement or electrohydrodynamic movement.
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Abstract
The invention relates generally to methods and apparatus for conducting analyses, particularly microfluidic devices for the detection of target analytes.
127 Citations
20 Claims
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1. A microfluidic device for the detection of a target analyte in a fluid sample, said device comprising:
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a) a solid support comprising a first plurality of electrodes configured for electrokinetic movement of at least a portion of the sample upon application of a voltage; and b) a detection module comprising a second plurality of electrodes configured for electrochemical detection of the target analyte, each comprising a binding ligand and a self-assembled monolayer; wherein the electrokinetic movement is electroosmotic movement or electrohydrodynamic movement. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11. A microfluidic device for the detection of a target analyte in a fluid sample, said device comprising:
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a) a solid support comprising a first plurality of electrodes configured for electrokinetic movement of at least a portion of the sample upon application of a voltage; and b) a detection module comprising a second plurality of electrodes configured for electrochemical detection of the target analyte, each comprising a binding ligand and a self-assembled monolayer; wherein the target analyte is a nucleic acid or a protein. - View Dependent Claims (12, 13, 14, 15, 16, 17, 18, 19, 20)
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Specification