Sample preparation devices and systems

• US 9,561,505 B2
• Filed: 09/11/2014
• Issued: 02/07/2017
• Est. Priority Date: 12/23/2011
• Status: Active Grant

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Malik, Imran R., Scherer, Axel
• Primary Examiner(s): Kim, Young J
• Application Number:
  US14/483,605
• Publication Number:
  US 20150024481A1
• Time in Patent Office: 880 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0867 Multiple inlets and one sample wells, e.g. mixing, dilution
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 2400/0478 pistons
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/0644 rotary valves
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502753 characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation separation in general B01D; microapparatus for analysis using electrophoresis G01N27/44791; sample preparation G01N1/28
• CPC Class Codes: B01L 3/502776 specially adapted for focusing or laminating flows
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: C12Q 1/68 involving nucleic acids
• CPC Class Codes: F17D 3/00 Arrangements for supervising or controlling working operations
• CPC Class Codes: G01N 2001/4088 filtration
• CPC Class Codes: G01N 35/1097 characterised by the valves valves in general F16K
• CPC Class Codes: Y10T 137/0318 : Processes
• CPC Class Codes: Y10T 137/8593 : Systems
• CPC Class Codes: Y10T 137/85978 : With pump

Abstract

Devices and system for preparing samples are described. Such devices can comprise fluidic chambers, reservoirs, and movable structures for controlling the movement of samples. The device can also comprise functional elements for performing specific operations.

Claims

1. A device for performing fluidic operations, comprising:

an adapter comprising at least one pair of ports;

a plurality of reservoirs fluidly connectable with the at least one pair of ports, the plurality of reservoirs configured to flow fluid from at least a first reservoir to at least a second reservoir;

a first structure associated with the adapter and displaceable with respect to the adapter, the first structure comprising a first channel arrangement configured to fluidly connect the at least first reservoir with the at least second reservoir, the first channel arrangement being alignable with a desired pair of ports through displacement of the first structure; and

a second structure layered on the first structure, the second structure comprising a second channel and the first structure further comprising a pair of fluidic vias adapted to be aligned with the second channel in the second structure such that when the fluid flows, the fluid flow from a first fluidic via of the pair, through the second channel, and through a respective second fluidic via of the pair.

2. The device according to claim 1, wherein the first channel arrangement comprises a module selected from the group consisting of:

a DNA binding matrix, a lysis, a plasma filter, a cell filter, a mixing filter, and a binding filter, whereby the module is located in a flow path of a fluid when the fluid flows through the first channel arrangement.

3. The device according to claim 1, wherein the displaceable first structure is rotatable in a radial direction of the device.

4. The device according to claim 2, wherein movement of the module through displacement of the first structure is synchronous with movement of the first channel arrangement comprising the module.

5. The device according to claim 1, wherein the first channel arrangement is one or more channels.

6. The device according to claim 1, wherein each reservoir of the plurality of reservoirs comprises a fluidic pressuring mechanism adapted to facilitate movement of the fluid from the first reservoir to the second reservoir, and vice versa.

7. The device according to claim 6, wherein the pressuring mechanism is selected from the group consisting of:

a piston, an internal actuator, an external actuator, and a pump.

8. The device according to claim 1, wherein the reservoirs are selected from the group consisting of:

syringes, syringes with pistons, tubes with a pinching mechanism, pouches, planar reservoirs and channels, reservoirs with flexible membranes, pipettes, and cartridges.