Nonaqueous backscattering interferometric methods
First Claim
Patent Images
1. A method for determining a characteristic property of a liquid sample comprising the steps of:
- a. providing an apparatus adapted for performing light scattering interferometry, the apparatus comprisingi. a substrate;
ii. a channel formed in the substrate capable of receiving the liquid sample to be analyzed;
iii. a light source for generating a light beam;
iv. a photodetector for receiving scattered light and generating intensity signals; and
v. at least one signal analyzer capable of receiving the intensity signals and determining therefrom one or more characteristic properties of the liquid sample; and
b. interrogating the liquid sample using backscattering interferometry, wherein the liquid sample comprises at least two binding partners in a solvent selected from ketone, aldehyde, alkane, chlorocarbon, sulfoxide, and acetonitrile, and wherein a molecular interaction between the binding partners is detected in the liquid sample via the interrogation.
2 Assignments
0 Petitions
Accused Products
Abstract
Disclosed are improved optical detection methods comprising multiplexed interferometric detection systems and methods for determining a characteristic property of a non-aqueous sample, together with various applications of the disclosed techniques.
325 Citations
20 Claims
-
1. A method for determining a characteristic property of a liquid sample comprising the steps of:
-
a. providing an apparatus adapted for performing light scattering interferometry, the apparatus comprising i. a substrate; ii. a channel formed in the substrate capable of receiving the liquid sample to be analyzed; iii. a light source for generating a light beam; iv. a photodetector for receiving scattered light and generating intensity signals; and v. at least one signal analyzer capable of receiving the intensity signals and determining therefrom one or more characteristic properties of the liquid sample; and b. interrogating the liquid sample using backscattering interferometry, wherein the liquid sample comprises at least two binding partners in a solvent selected from ketone, aldehyde, alkane, chlorocarbon, sulfoxide, and acetonitrile, and wherein a molecular interaction between the binding partners is detected in the liquid sample via the interrogation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
-
-
9. A method for determining a molecular interaction comprising the steps of:
-
a. providing a substrate having a channel formed therein for reception of a liquid sample to be analyzed; b. introducing the liquid sample to be analyzed into the channel, wherein the liquid sample comprises at least two binding partners in a solvent selected from hydrocarbon and acetonitrile; c. directing a light beam from a light source onto the substrate such that the light beam is incident on at least a portion of the liquid sample to generate scattered light through reflective and refractive interaction of the light beam with a substrate/channel interface, and the liquid sample, wherein the scattered light comprising interference fringe patterns including a plurality of spaced light bands whose positions shift in response to changes in the refractive index of the sample; d. detecting positional shifts in the light bands; and e. determining the molecular interaction between the binding partners in the liquid sample from the positional shifts of the light bands in the interference fringe patterns. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
-
-
17. A method comprising the steps of:
-
a. interrogating a liquid sample comprising at least two binding partners in a solvent selected from hydrocarbon, ketone, aldehyde, alkane, chlorocarbon, sulfoxide, and acetonitrile by using backscattering interferometry, and b. detecting a change in refractive index of the liquid sample due to a molecular interaction between the binding partners. - View Dependent Claims (18, 19, 20)
-
Specification