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Sandwich assays in droplets

  • US 9,562,897 B2
  • Filed: 09/30/2011
  • Issued: 02/07/2017
  • Est. Priority Date: 09/30/2010
  • Status: Active Grant
First Claim
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1. A method for measuring the quantity of target analytes, the method comprising:

  • forming a plurality of fluidic compartmentalized portions, each comprising;

    a target analyte from a sample, a first binding agent specific for a first binding region on the target analyte and comprising a target identifier, and a second binding agent specific for a second binding region on the target analyte, wherein the second binding agent comprises a functionalized portion for immobilization to a solid support;

    producing in the one or more compartmentalized portions, one or more complexes each comprising the first and second binding agents bound to the target analyte;

    disrupting and releasing contents of the plurality of compartmentalized portions;

    immobilizing the one or more complexes on a solid support through the functionalized portion of the second binding agent to form one or more immobilized complexes;

    separating the immobilized complexes from the other released contents; and

    detecting the target identifiers of the separated immobilized complexes, thereby identifying the quantity of the target analyte.

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