Compositions and methods for diagnosing and treating hyperthyroidism in companion animals

• US 9,566,333 B2
• Filed: 12/06/2012
• Issued: 02/14/2017
• Est. Priority Date: 12/19/2011
• Status: Active Grant

• Current Assignee: Hill's Pet Nutrition Incorporated (Colgate-Palmolive Company)
• Original Assignee: Hill's Pet Nutrition Incorporated (Colgate-Palmolive Company)
• Inventors: Al-Murrani, Samer, Brockman, Jeffrey
• Primary Examiner(s): PAK, MICHAEL D
• Application Number:
  US14/366,667
• Publication Number:
  US 20150023968A1
• Time in Patent Office: 1,531 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: A23K 20/153 Nucleic acids; Hydrolysis products or derivatives thereof
• CPC Class Codes: A23K 50/40 for carnivorous animals, e.g. cats or dogs
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61K 39/3955 against proteinaceous materials, e.g. enzymes, hormones, lymphokines
• CPC Class Codes: A61K 47/50 the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
• CPC Class Codes: A61K 49/00 Preparations for testing in vivo
• CPC Class Codes: A61P 5/14 of the thyroid hormones, e.g. T3, T4
• CPC Class Codes: C07K 14/473 alpha-Glycoproteins
• CPC Class Codes: C07K 14/705 Receptors; Cell surface antigens; Cell surface determinants tumour specific antigens C07K14/4748
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/54 F(ab')2
• CPC Class Codes: C07K 2317/55 Fab or Fab'
• CPC Class Codes: C07K 2317/76 Antagonist effect on antigen, e.g. neutralization or inhibition of binding
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 2310/14 interfering N.A.
• CPC Class Codes: C12Q 1/6883 for diseases caused by alterations of genetic material
• CPC Class Codes: C12Q 2600/158 Expression markers
• CPC Class Codes: G16B 50/00 ICT programming tools or database systems specially adapted for bioinformatics

Abstract

An isolated DNA molecule comprising a fragment of the gene encoding the feline NIS is disclosed as well as methods of use thereof. Also provided are methods for rational diet design of food composition suitable for administration to feline companion animals afflicted with hyperthyroidism, comprising (a) accessing at least one database that comprises a first data set relating functional gene profile of a biofluid or tissue sample from an animal to physiological condition of the animal, where the functional gene profile is that of the feline NIS gene; (b) accessing at least one database that comprises a second data set relating effects of bioactive dietary components on the functional gene profile; (c) using an algorithm drawing on these data sets, processing input data defining physiological condition to derive a nutritional formula promoting wellness of a feline companion animal afflicted with hyperthyroidism; and (d) preparing a food composition based on the nutritional formula.

Claims

1. An isolated cDNA molecule comprising (a) a nucleotide sequence that encodes a protein having the amino acid sequence of SEQ ID NO:

2, or (b) a nucleotide sequence that is completely complementary to the nucleotide sequence of (a).

4. A recombinant DNA vector comprising an insert, wherein the insert comprises the cDNA molecule of claim 1.

5. A transformed cell comprising the recombinant DNA vector of claim 4.

6. The recombinant DNA vector of claim 4, further comprising a nucleic acid sequence encoding a reporter polypeptide.

7. The recombinant DNA vector of claim 6, further comprising a thyroid response element, wherein the thyroid response element is in operative association with the reporter polypeptide.

8. The cDNA molecule of claim 1, further comprising a labeling moiety.

11. The cDNA molecule of claim 8, wherein the labeling moiety is a fluorophore or streptavidin.

2. An isolated cDNA molecule comprising at least 70 contiguous nucleotides of SEQ ID NO:

1 or at least 70 contiguous nucleotides completely complementary to the nucleotide sequence of SEQ ID NO;

1.

3. The isolated cDNA molecule of claim 2, wherein the 70 contiguous nucleotides are within a coding sequence of the feline sodium/iodide symporter protein (NIS protein).

9. The cDNA molecule of claim 2, further comprising a labeling moiety.

10. The cDNA molecule of claim 3, further comprising a labeling moiety.

12. The cDNA molecule of claim 9, wherein the labeling moiety is a fluorophore or streptavidin.

13. The cDNA molecule of claim 10, wherein the labeling moiety is a fluorophore or streptavidin.