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Mono- and di-peg IL-10 production; and uses

  • US 9,566,345 B2
  • Filed: 01/06/2016
  • Issued: 02/14/2017
  • Est. Priority Date: 12/17/2008
  • Status: Active Grant
First Claim
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1. A method of producing a mixture of mono- and di-pegylated interleukin-10 (IL-10), wherein at least one PEG molecule is covalently attached to at least one amino acid residue of at least one subunit of IL-10, comprising:

  • a) reacting 1 mg/ml to 12 mg/ml of IL-10 with an activated PEG-linker in the presence of 25 mM to 35 mM of a reducing agent, wherein the PEG-linker is selected from the group consisting of succinimidylcarbonate-PEG, PEG-butyraldehyde, PEG-pentaldehyde, PEG-amido-propionaldehyde, PEG-urethano-propioaldehyde, and PEG-propylaldehyde; and

    b) purifying the mixture of mono- and di-pegylated IL-10.

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