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In situ oriented immobilization of proteins on a support

  • US 9,567,378 B2
  • Filed: 06/03/2011
  • Issued: 02/14/2017
  • Est. Priority Date: 06/03/2010
  • Status: Active Grant
First Claim
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1. A method of directing in situ oriented immobilization of a protein on a support, said method comprising:

  • providing a support;

    contacting the support with a solution comprising;

    a protein comprising a coupling moiety, wherein the protein is a recombinant fusion protein and the coupling moiety comprises glutathione-S-transferase (GST) anda glutathione molecule comprising a first group reactive with the support and a second group reactive with the coupling moiety,wherein the glutathione molecule binds (i) the support at the first group and (ii) the coupling moiety at the second group, thereby immobilizing and orienting, in situ, the protein on the support.

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