Bioreactors comprising fungal strains

• US 9,567,556 B2
• Filed: 03/06/2015
• Issued: 02/14/2017
• Est. Priority Date: 05/07/2010
• Status: Active Grant

• Current Assignee: Menon Renewable Products, Inc. (Menon & Associates, Inc.)
• Original Assignee: Menon Renewable Products, Inc. (Menon & Associates, Inc.)
• Inventors: Menon, Suresh M., Orchard, Samantha S., Badger, Jessica, Lipson, David, Guidi, Sara, Newman, David
• Primary Examiner(s): Shen, Bin
• Application Number:
  US14/641,159
• Publication Number:
  US 20150175947A1
• Time in Patent Office: 711 Days
• US Class Current: 1/1
• CPC Class Codes: A01N 63/36 Penicillium
• CPC Class Codes: A23K 10/12 by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
• CPC Class Codes: A23K 10/30 from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms obtained by microbiological or biochemical processes, e.g. using yeasts or enzymes, A23K10/10
• CPC Class Codes: A23K 20/142 Amino acids; Derivatives thereof
• CPC Class Codes: A23K 20/147 Polymeric derivatives, e.g. peptides or proteins
• CPC Class Codes: A23K 20/158 Fatty acids; Fats; Products containing oils or fats
• CPC Class Codes: A23K 40/20 by moulding, e.g. making cakes or briquettes
• CPC Class Codes: A23K 50/10 for ruminants
• CPC Class Codes: A23K 50/42 Dry feed
• CPC Class Codes: A23K 50/75 for poultry
• CPC Class Codes: A23K 50/80 for aquatic animals, e.g. fish, crustaceans or molluscs
• CPC Class Codes: A23L 31/00 Edible extracts or preparations of fungi; Preparation or treatment thereof
• CPC Class Codes: A23L 33/10 using additives addition of substantially indigestible substances A23L33/21
• CPC Class Codes: A23L 33/12 Fatty acids or derivatives thereof
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: C11B 1/025 by enzymes or microorganisms, living or dead
• CPC Class Codes: C11B 1/10 by extracting
• CPC Class Codes: C11C 3/003 by esterification of fatty acids with alcohols C11C3/02 takes precedence
• CPC Class Codes: C12M 21/12 for producing fuels or solvents C12M21/04 takes precedence; liquid carbonaceous fuels C10L1/00, solid fuels C10L5/00
• CPC Class Codes: C12M 25/02 Membranes; Filters filters or filtration in general B01D24/00-B01D41/00
• CPC Class Codes: C12M 27/02 : Stirrer or mobile mixing elements
• CPC Class Codes: C12M 27/20 : Baffles; Ribs; Ribbons; Auger vanes
• CPC Class Codes: C12N 1/14 : Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12N 1/145 : Fungal isolates
• CPC Class Codes: C12P 2203/00 : Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
• CPC Class Codes: C12P 5/005 : aromatic naphthacene C12P29/00
• CPC Class Codes: C12P 5/02 : acyclic C12P5/007 takes precedence
• CPC Class Codes: C12P 7/6458 : by transesterification, e.g. interesterification, ester interchange, alcoholysis or acidolysis
• CPC Class Codes: C12P 7/6463 : obtained from glyceride producing microorganisms, e.g. single cell oil
• CPC Class Codes: C12P 7/6481 : Phosphoglycerides phosphoglycerides having carboxylic acids with less than seven carbon atoms C12P7/62
• CPC Class Codes: C12R 2001/645 : Fungi ; Processes using fungi
• CPC Class Codes: C12R 2001/80 : Penicillium
• CPC Class Codes: Y02A 40/818 : Alternative feeds for fish, e.g. in aquacultures
• CPC Class Codes: Y10S 426/805 : Pet food for dog, cat, bird, or fish
• CPC Class Codes: Y10S 426/807 : Poultry or ruminant feed

Abstract

Disclosed herein are compositions comprising an isolated cellulose degrading fungus. Also disclosed are culture compositions and bioreactor compositions comprising the cellulose degrading fungus. Further described herein are filtration and extraction devices comprising the cellulose degrading fungus. Still further disclosed are bioprocessing facilities for and methods for producing co-products resulting from one or more bioprocesses of the cellulose degrading fungus.

Claims

1. A method for producing an admixture for animal feed comprising:

combining a fungal biomass with additional nutrition thereby producing an admixture, wherein the admixture is suitable for animal consumption and the animal feed does not contain whey, thereby producing an admixture for animal feed, wherein the fungal biomass comprises a fungus selected from the group consisting of;

a fungus having a 5.8S ribosomal RNA gene sequence with at least 98% nucleotide sequence identity to the nucleic acid of SEQ ID NO;

1;

a fungus having an ITS1 sequence with at least 98% sequence identity to SEQ ID NO;

2;

a fungus having an ITS2 sequence with at least 98% sequence identity to SEQ ID NO;

3;

a fungus having a 28S ribosomal RNA gene sequence with at least 98% sequence identity to SEQ ID NO;

4, Penicillium menonorum and a species being the same as NRRL deposit Accession No;

50410, wherein the additional nutrition comprises at least one nutrient selected from the group consisting of soybean meal, wheat flour, feed peas, fishmeal, herring, wheat gluten, squid meal, hill, fish oil, lecithin, mono calcium phosphate, a mineral, a vitamin, cholesterol, and combinations thereof.

2. The method of claim 1, wherein the fungal biomass is produced by:

a) inoculating a medium with the degrading fungus;

b) fermenting the inoculated medium under aerobic conditions thereby producing a fungal biomass; and

c) recovering the fungal biomass.

3. The method of claim 2, wherein the medium comprises sugars, cane juice or its condensates or dry solid evaporate, or whey.

4. The method of claim 2, wherein the medium comprises a cellulosic feedstock.

5. The method of claim 4, wherein the cellulosic feedstock comprises at least one of:

grains, stover, forage, grasses, oilseed crops, nut shells, nut hulls, fruit pomace, plant waste, algae, wood, wood byproducts, wood chips, bark, paper, paper products, animal manure, food waste, yard waste, almond hulls, grape pomace, agriculture waste, and sawdust.

6. The method of claim 2, wherein the medium comprises a feedstock, wherein said feedstock is a solid feedstock, a hydrolysate in a liquid, or a combination thereof.

7. The method of claim 6, further comprising pretreating the solid feedstock prior to inoculating the medium.

8. The method of claim 7, wherein pretreating comprises mechanical pretreatment, thermal-chemical pretreatment, heat sterilization, ultraviolet irradiation, pasteurization, filtration, separation or a combination thereof.

9. The method of claim 8, wherein pretreating comprises mechanical pretreatment to increase the ratio of surface area to volume of the feedstock and subsequent filtration of the mechanically pretreated feedstock.

10. The method of claim 2, wherein the cellulose degrading fungus is a species being the same as NRRL deposit Accession No:

50410.

11. The method of claim 2, wherein the admixture comprises 1% to 90% fungal biomass by weight.

12. The method of claim 11, wherein the admixture comprises 5% to 50% fungal biomass by weight.

13. The method of claim 12, wherein the fungal biomass comprises 10% to about 50% cdw triacylglyceride.

14. The method of claim 13, wherein the fungal biomass comprises at least about 25% to about 50% triacylglyceride by dry weight.

15. The method of claim 2, wherein the fungal biomass comprises biomass harvested at either a time that an available nitrogen ingredient is exhausted, or a time when log-phase growth terminates.

16. The method of claim 2, wherein the medium comprises biological matter comprising at least one of:

a cellulosic carbon source or a lysate or a hydrolysate thereof, a hemicellulosic carbon source or a lysate or a hydrolysate thereof, and a lignocellulosic carbon source or a lysate or a hydrolysate thereof.

17. The method of claim 1, wherein the fungal biomass comprises greater than about 25% cell dry weight (cdw) crude protein.

18. The method of claim 1, wherein the fungal biomass comprises greater than about 10% cell dry weight (cdw) lipid.

19. The method of claim 1, wherein the admixture comprises at least 3% of the animal feed.

20. The method of claim 1, wherein the animal feed comprises 35% crude protein, 6.5-6.7% crude fat, and 3.1 to 6.3% crude fiber.

21. The method of claim 1, wherein the cellulose degrading fungus is a species being the same as NRRL deposit Accession No:

50410.

22. The method of claim 1, wherein said fungus has a 5.8S ribosomal RNA gene sequence with at least 98% nucleotide sequencing identity to the nucleic acid of SEQ ID NO:

1.

23. The method of claim 1, wherein said fungus has a 5.8S ribosomal RNA gene sequence of SEQ ID NO:

1.

24. The method of claim 1, wherein said fungus has an ITS1 sequence with at least 98% sequence identity to SEQ ID NO:

2.

25. The method of claim 1, wherein said fungus has an ITS1 sequence of SEQ ID NO:

2.

26. The method of claim 1, wherein said fungus has an ITS2 sequence with at least 98% sequence identity to SEQ ID NO:

3.

27. The method of claim 1, wherein said fungus has an ITS2 sequence of SEQ ID NO:

3.

28. The method of claim 1, wherein said fungus has a 28S ribosomal RNA gene sequence with at least 98% sequence identity to SEQ ID NO:

4.

29. The method of claim 1, wherein said fungus has a 28S ribosomal RNA gene sequence of SEQ ID NO:

4.

30. The method of claim 1, wherein the fungus is Penicillium menonorum.

31. The method of claim 1, wherein said fungal biomass comprises a fatty acid.

32. The method of claim 31, wherein the fatty acid is selected from the group consisting of myristic acid, pentadecylic acid, palmitic acid, margaric acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachic acid, behenic acid, lignoceric acid and combinations thereof.

33. The method of claim 32, wherein the fatty acid is selected from the group consisting of palmitic acid, oleic acid, and linoleic acid.

34. The method of claim 32, wherein the fatty acid is linolenic acid.

35. The method of claim 34, wherein the fatty acid is present as TAG, and at least 3.0% of the TAG is linolenic acid.