Compositions for linking DNA-binding domains and cleavage domains
First Claim
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1. A fusion protein comprisinga DNA-binding domain having an N-terminus and a C-terminus, wherein the DNA-binding domain binds to a nucleotide target site;
- a FokI cleavage domain having an N-terminus and a C-terminus; and
a linker between the C-terminus of the DNA-binding domain and the N-terminus of the cleavage domain, wherein the linker comprises a sequence selected from the group consisting of PKPAN (SEQ ID NO;
31), RARPLN (SEQ ID NO;
32);
PMPPLA (SEQ ID NO;
33) or PPPRP (SEQ ID NO;
34).
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Abstract
Disclosed herein are compositions for linking DNA binding domains and cleavage domains (or cleavage half-domains) to form non-naturally occurring nucleases. Also described are methods of making and using compositions comprising these linkers.
94 Citations
16 Claims
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1. A fusion protein comprising
a DNA-binding domain having an N-terminus and a C-terminus, wherein the DNA-binding domain binds to a nucleotide target site; -
a FokI cleavage domain having an N-terminus and a C-terminus; and a linker between the C-terminus of the DNA-binding domain and the N-terminus of the cleavage domain, wherein the linker comprises a sequence selected from the group consisting of PKPAN (SEQ ID NO;
31), RARPLN (SEQ ID NO;
32);
PMPPLA (SEQ ID NO;
33) or PPPRP (SEQ ID NO;
34). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification