Quantitative multi-spectral opto-acoustic tomography (MSOT) of tissue biomarkers
First Claim
1. A method of multi-spectral opto-acoustic tomography (MSOT) imaging of a target tissue including at least one target tissue biomarker, the method comprising:
- illuminating the target tissue using several different illumination wavelengths;
detecting pressure signals from the at least one target tissue biomarker with a detector, wherein the pressure signals are produced in the target tissue in response to said illuminating the target tissue; and
reconstructing a quantitative tomographic image of a spatial distribution of a concentration of the at least one target tissue biomarker in the target tissue, said reconstructing of the quantitative tomographic image comprising;
applying an inversion scheme to the pressure signals to provide a reconstructed optoacoustic image ψ
k(λ
) at each of the different illumination wavelengths;
separating contributions of a light fluence Uk(λ
) and an absorption coefficient μ
k(λ
) to the reconstructed image ψ
k(λ
) at each of the illumination wavelengths using a blind source separation method, whereby the absorption coefficient μ
k(λ
) is obtained at each of the illumination wavelengths; and
using at least one spectral processing scheme to resolve the at least one target tissue biomarker, wherein the spectral processing scheme processes, on a pixel-by-pixel basis, the absorption coefficient μ
k(λ
) obtained at each of the illumination wavelengths to resolve the spatial distribution of the concentration of the at least one target tissue biomarker.
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Abstract
A method of multi-spectral opto-acoustic tomography (MSOT) imaging of a target tissue including a target tissue biomarker includes illuminating the target tissue with an illumination device emitting at least one pulsed illumination pattern at several illumination wavelengths, detecting pressure signals from the target tissue biomarker with a detector device, wherein the pressure signals being produced in the target tissue are in response to the illumination, and reconstructing a quantitative tomographic image of a distribution of the target tissue biomarker in the target tissue, wherein the pressure signals are analyzed using a photon propagation model which depends on an illuminating light fluence in the target tissue and on the illumination wavelengths, at least one spectral processing scheme, and an inversion scheme providing the tomographic image.
110 Citations
27 Claims
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1. A method of multi-spectral opto-acoustic tomography (MSOT) imaging of a target tissue including at least one target tissue biomarker, the method comprising:
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illuminating the target tissue using several different illumination wavelengths; detecting pressure signals from the at least one target tissue biomarker with a detector, wherein the pressure signals are produced in the target tissue in response to said illuminating the target tissue; and reconstructing a quantitative tomographic image of a spatial distribution of a concentration of the at least one target tissue biomarker in the target tissue, said reconstructing of the quantitative tomographic image comprising; applying an inversion scheme to the pressure signals to provide a reconstructed optoacoustic image ψ
k(λ
) at each of the different illumination wavelengths;separating contributions of a light fluence Uk(λ
) and an absorption coefficient μ
k(λ
) to the reconstructed image ψ
k(λ
) at each of the illumination wavelengths using a blind source separation method, whereby the absorption coefficient μ
k(λ
) is obtained at each of the illumination wavelengths; andusing at least one spectral processing scheme to resolve the at least one target tissue biomarker, wherein the spectral processing scheme processes, on a pixel-by-pixel basis, the absorption coefficient μ
k(λ
) obtained at each of the illumination wavelengths to resolve the spatial distribution of the concentration of the at least one target tissue biomarker. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 23)
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15. An imaging device for multi-spectral opto-acoustic tomography (MSOT) imaging of a target tissue including at least one target tissue biomarker, the imaging device comprising:
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an illumination device that comprises a light source or a light guide that transmits light from a light source, the illumination device being configured to illuminate the target tissue with at least one pulsed illumination pattern at several different illumination wavelengths that are absorbed by the target tissue biomarker; a detector that detects pressure signals produced from the at least one target tissue biomarker in the target tissue in response to illumination of the target tissue via the illumination device; and one or more processors that are configured to reconstruct a quantitative tomographic image of a spatial distribution of a concentration of the at least one target tissue biomarker in the target tissue by; applying an inversion scheme to the pressure signals to provide a reconstructed optoacoustic image ψ
k(λ
) at each of the different illumination wavelengths;separating contributions of a light fluence Uk(λ
) and an absorption coefficient μ
k(λ
) to the reconstructed image ψ
k(λ
) at each of the illumination wavelengths using a blind source separation method, whereby the absorption coefficient μ
k(λ
) is obtained at each of the illumination wavelengths; andapplying a spectral processing scheme to resolve the at least one target tissue biomarker, wherein the spectral processing scheme processes, on a pixel-by-pixel basis, the absorption coefficient μ
k(λ
) obtained at each of the illumination wavelengths to resolve the spatial distribution of the concentration of the at least one target tissue biomarker. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 24)
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25. A method of multi-spectral opto-acoustic tomography (MSOT) imaging of a target tissue including at least one target tissue biomarker, the method comprising:
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illuminating the target tissue using several different illumination wavelengths; detecting pressure signals from the at least one target tissue biomarker with a detector, wherein the pressure signals are produced in the target tissue in response to said illuminating the target tissue; and reconstructing a quantitative tomographic image of a spatial distribution of a concentration of the at least one target tissue biomarker in the target tissue, said reconstructing of the quantitative tomographic image comprising; applying an inversion scheme to the pressure signals to provide a reconstructed optoacoustic image ψ
k(λ
) at each of the different illumination wavelengths;separating contributions of a light fluence Uk(λ
) and an absorption coefficient μ
k(λ
) to the reconstructed image ψ
k(λ
) at each of the illumination wavelengths using a blind source separation method, whereby the absorption coefficient μ
k(λ
) is obtained at each of the illumination wavelengths; andusing at least one spectral processing scheme to resolve the at least one target tissue biomarker, wherein the spatial distribution of the concentration of the at least one target tissue biomarker and concentrations of background chromophores are reconstructed for every pixel k from linear equations μ
k(λ
)=α
MM(λ
)cMMk+α
1(λ
)c1k+α
2(λ
)c2k+. . . for each of the illumination wavelengths λ
, wherein μ
k(λ
) is the absorption coefficient obtained at each of the illumination wavelengths λ
, wherein α
MM(λ
), α
1(λ
), α
2(λ
), . . . are molar extinction spectra of the target tissue biomarker and the background chromophores, respectively, wherein cMMk is the concentration of the target tissue biomarker, and wherein c1k, c2k, . . . are the concentrations of the background chromophores at every pixel k. - View Dependent Claims (26)
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27. An imaging device for multi-spectral opto-acoustic tomography (MSOT) imaging of a target tissue including at least one target tissue biomarker, the imaging device comprising:
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an illumination device that comprises a light source or a light guide that transmits light from a light source, the illumination device being configured to illuminate the target tissue with at least one pulsed illumination pattern at several different illumination wavelengths that are absorbed by the target tissue biomarker; a detector that detects pressure signals produced from the at least one target tissue biomarker in the target tissue in response to illumination of the target tissue via the illumination device; and one or more processors that are configured to reconstruct a quantitative tomographic image of a spatial distribution of a concentration of the at least one target tissue biomarker in the target tissue by; applying an inversion scheme to the pressure signals to provide a reconstructed optoacoustic image ψ
k(λ
) at each of the different illumination wavelengths;separating contributions of a light fluence Uk(λ
) and an absorption coefficient μ
k(λ
) to the reconstructed image ψ
k(λ
) at each of the illumination wavelengths using a blind source separation method, whereby the absorption coefficient μ
k(λ
) is obtained at each of the illumination wavelengths; andapplying a spectral processing scheme to resolve the at least one target tissue biomarker, wherein the spatial distribution of the concentration of the at least one target tissue biomarker and concentrations of background chromophores are reconstructed for every pixel k from linear equations μ
k(λ
)=α
MM(λ
)cMMk+α
1(λ
)c1k+α
2(λ
)c2k+. . . for each of the illumination wavelengths λ
, wherein μ
k(λ
) is the absorption coefficient obtained at each of the illumination wavelengths λ
, wherein α
MM(λ
), α
1(λ
), α
2(λ
), . . . are molar extinction spectra of the target tissue biomarker and the background chromophores, respectively, wherein cMMk is the concentration of the target tissue biomarker, and wherein c1k, c2k, . . . are the concentrations of the background chromophores at every pixel k.
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Specification