Fluidics device allowing fluid flow between a plurality of wells
First Claim
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1. A fluidics device comprising:
- a unitarily constructed tray configured to rest on a substantially flat surface including;
a dosing well positioned upstream from a plurality of wells aligned in a row for containing a respective host fluid,wherein each of the plurality of wells defines a cavity having a bottom surface, a wall surface extending from the bottom surface and an open side defined by a circumference of the wall surface,wherein, when the tray rests on the flat surface, each of the open sides is in a step-down position relative to the open side of each adjacent upstream well and the wall surface is substantially perpendicular to the flat surface; and
one or more channels extending between the open side of adjacent upstream wells and the open side of the adjacent downstream wells to define a channel fluid flow path there between.
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Abstract
Disclosed are fluidics devices and assemblies allowing for fluid flow between a plurality of wells. The fluidics devices and assemblies that are provided mimic in vivo tissue environments by allowing for initially segregated tissue cultures that can then be linked through fluid flow to measure integrated tissue response. The devices and assemblies provide a pumpless system using surface tension, gravity, and channel geometries. By linking human tissue functional systems to better simulate in vivo feedback and response signals between the tissues, the need for testing in animals can be minimized.
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Citations
33 Claims
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1. A fluidics device comprising:
a unitarily constructed tray configured to rest on a substantially flat surface including; a dosing well positioned upstream from a plurality of wells aligned in a row for containing a respective host fluid, wherein each of the plurality of wells defines a cavity having a bottom surface, a wall surface extending from the bottom surface and an open side defined by a circumference of the wall surface, wherein, when the tray rests on the flat surface, each of the open sides is in a step-down position relative to the open side of each adjacent upstream well and the wall surface is substantially perpendicular to the flat surface; and one or more channels extending between the open side of adjacent upstream wells and the open side of the adjacent downstream wells to define a channel fluid flow path there between. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26)
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27. An assembly for allowing fluid flow between a plurality of wells comprising:
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a first fluidics device comprising; a dosing well positioned upstream from a plurality of wells aligned in a row for containing a respective host fluid, wherein each of the plurality of wells defines a cavity having a bottom surface a wall surface extending from the bottom surface and an open side defined by a circumference of the wall surface, wherein each of the open sides is in a step-down position relative to the open side of each adjacent upstream well; and one or more channels extending between the open side of adjacent upstream wells and the open side of the adjacent downstream wells to define a channel fluid flow path there between; and a reservoir tray configured for nesting engagement on top of the first fluidics device, the reservoir tray comprising; at least one chamber for containing a respective chamber fluid; and a reservoir aperture defined in a floor of the at least one chamber and configured such that the reservoir aperture is positioned above the dosing well of the first fluidics device when in nesting engagement with the first fluidics device, wherein the floor of the at least one chamber is angled and the reservoir aperture is defined at a lower portion of the floor of the at least one chamber such that the chamber fluid flows through the reservoir aperture into the dosing well of the first fluidics device when the reservoir tray and the first fluidics device are nestably engaged. - View Dependent Claims (28, 29, 30, 31, 32, 33)
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Specification