System and methods for monitoring the amplification and dissociation behavior of DNA molecules

• US 9,573,132 B2
• Filed: 11/30/2006
• Issued: 02/21/2017
• Est. Priority Date: 06/30/2006
• Status: Active Grant

• Current Assignee: Canon USA, Inc. (Canon Inc.)
• Original Assignee: Canon USA, Inc. (Canon Inc.)
• Inventors: Hasson, Kenton C., Dale, Gregory A.
• Primary Examiner(s): MUMMERT, STEPHANIE KANE
• Application Number:
  US11/606,006
• Publication Number:
  US 20080003593A1
• Time in Patent Office: 3,736 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/10 Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2300/0627 Sensor or part of a sensor is integrated
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/18 Means for temperature control
• CPC Class Codes: B01L 2300/1811 using electromagnetic induction heating
• CPC Class Codes: B01L 2300/1822 using Peltier elements
• CPC Class Codes: B01L 2300/1827 using resistive heater
• CPC Class Codes: B01L 2300/1877 using chemical reactions
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 7/52 with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples amplification or hybridisation processes per se C12Q1/68; controlling sequential reactions for synthesis B01J19/0046
• CPC Class Codes: B01L 7/525 with physical movement of samples between temperature zones
• CPC Class Codes: C12Q 1/686 Polymerase chain reaction [PCR]
• CPC Class Codes: C12Q 2561/113 Real time assay
• CPC Class Codes: C12Q 2565/629 being a microfluidic device
• CPC Class Codes: G01N 21/71 thermally excited
• CPC Class Codes: G01N 35/08 : using a stream of discrete samples flowing along a tube system, e.g. flow injection analysis
• CPC Class Codes: G06T 2207/10004 : Still image; Photographic image
• CPC Class Codes: G06T 2207/30072 : Microarray; Biochip, DNA array; Well plate
• CPC Class Codes: G06T 7/0012 : Biomedical image inspection
• CPC Class Codes: G06T 7/11 : Region-based segmentation
• CPC Class Codes: Y10T 436/115831 : Condition or time responsive
• CPC Class Codes: Y10T 436/117497 : with a continuously flowing sample or carrier stream

Abstract

The present invention relates to systems and methods for monitoring the amplification of DNA molecules and the dissociation behavior of the DNA molecules. A method according to one embodiment of the invention may include the steps of: forcing a sample of a solution containing real-time PCR reagents to move though a channel; and while the sample is moving through an analysis region of the channel, performing the steps of: (a) cycling the temperature of the sample until the occurrence of a predetermined event; (b) after performing step (a), causing the sample'"'"'s temperature to gradually increase from a first temperature to a second temperature; and (c) while the step of gradually increasing the sample'"'"'s temperature is performed, using an image sensor to monitor emissions from the sample.

Claims

1. A method, comprising:

(a) introducing into a microchannel at least one bolus containing nucleic acid;

(b) forcing the bolus to move through the microchannel;

(c) while the bolus is moving through the microchannel, (i) causing a thermal generating apparatus to cycle the temperature of the bolus to amplify nucleic acid contained in the bolus, wherein the thermal generating apparatus sequentially controls temperature during amplification and thermal denaturation, the thermal denaturation being performed after the amplification is completed and (ii) at least once per temperature cycle determining whether a predetermined emission intensity threshold has been met, wherein the step of determining whether the predetermined emission intensity threshold has been met comprises using an image sensor to capture an image of the bolus and processing the captured image data to determine the intensity of light emitted from the bolus;

(d) in response to determining that the predetermined emission intensity threshold has not been met, then repeating step (c); and

(e) in response to determining that the predetermined emission intensity threshold has been met, then, causing said thermal generating apparatus to cease the temperature cycling and causing said thermal generating apparatus to gradually increase the temperature of the bolus, while the bolus is still moving through the microchannel, causing thermal denaturation of dsDNA within the bolus to transition to ssDNA and using the image sensor to capture images of the bolus.

2. The method of claim 1, wherein the step of determining whether the predetermined emission intensity threshold has been met further comprises (a) comparing a value representing the determined intensity to a threshold value or (b) determining the rate of change in the intensity of the emissions from the bolus.

3. The method of claim 2, further comprising initiating a process for causing the dsDNA within the bolus to transition to ssDNA in response to determining that the result of the comparison indicates that the value representing the determined intensity is greater than the threshold value.

4. The method of claim 1, wherein the temperature is increased at or about a constant rate.

5. The method of claim 4, wherein the constant rate is between about 0.1 and 2 degree Celsius per second.

6. The method of claim 1, wherein the step of using the image sensor to capture an image of the bolus comprises determining a region of interest within the field of view of the image sensor and then reading out only those pixels of the image sensor that correspond to the region of interest.

7. The method of claim 1, wherein step (c) further comprises

(iii) determining whether (a) the bolus has been exposed to at least a predetermined number of PCR temperature cycles, (b) at least a predetermined amount of time has elapsed since particular point in time, and/or (c) the bolus has entered a predefined region of the microchannel.

8. A method for use in a system comprising a substrate comprising a channel, the method comprising:

forcing a sample of a solution containing real-time PCR reagents to move though the channel; and

while the sample is moving through an analysis region of the channel performing the steps of;

(a) causing thermal generating apparatus to cycle the temperature of the sample, wherein the thermal generating apparatus sequentially controls temperature during amplification and thermal denaturation, the thermal denaturation being performed after the amplification is completed;

(b) at least once per temperature cycle, determining whether nucleic acid in the sample has been sufficiently amplified by using an image sensor to capture an image of the bolus and processing the captured image data to determine the intensity of light emitted from the bolus;

(c) in response to determining that a rate of change in the intensity of light emitted from the bolus is less than a predetermined threshold, causing said thermal generating apparatus to cease the cycling of the temperature of the sample and causing said thermal generating apparatus to gradually increase the sample'"'"'s temperature from a first temperature to a second temperature to cause thermal denaturation of the nucleic acid; and

(d) while the step of gradually increasing the sample'"'"'s temperature is performed, using an image sensor to monitor emissions from the sample.

9. The method of claim 8, wherein the step of determining whether the nucleic acid in the sample has been sufficiently amplified comprises determining the intensity of light emitted from the sample and comparing the determined intensity to an intensity threshold value.

10. The method of claim 9, wherein the step of using the image sensor to capture an image of the sample comprises determining a region of interest within the field of view of the image sensor and then reading out only those pixels of the image sensor that correspond to the region of interest.

11. The method of claim 8, wherein the step of cycling the temperature of the sample comprises performing the following steps one or more times:

(i) holding the temperature of the sample at or about a first temperature for at least a first period of time;

(ii) after step (i), using the thermal generating apparatus to heat the temperature of the sample from the first temperature to a second temperature;

(iii) after step (ii), holding the temperature of the sample at or about the second temperature for at least a second period of time;

(iv) after step (iii), using the thermal generating apparatus to heat the temperature of the sample from the second temperature to a third temperature;

(v) after step (iv);

holding the temperature of the sample at or about the third temperature for at least a third period of time.

12. The method of claim 8, wherein the step of gradually increasing the temperature of the sample from the first temperature to the second temperature comprises increasing the temperature at or about a constant rate.

13. The method of claim 12, wherein the constant rate is between about 0.1 and 1 degrees Celsius per second.

14. The method of claim 13, wherein increasing the temperature at or about a constant rate comprises increasing the temperature at said rate for at least about 1 minute.

15. The method of claim 8, further comprising the step of keeping a record of the number of temperature cycles to which the sample is exposed.

16. The method of claim 15, further comprising the step of determining whether the sample has been exposed to at least a predetermined number of temperature cycles.

17. The method of claim 8, further comprising the step of determining whether at least a predetermined amount of time has elapsed since a particular point in time.

18. The method of claim 8, further comprising the step of determining whether the sample has entered a predefined region of the channel.

19. A method, comprising:

(a) introducing into a microchannel at least one bolus containing nucleic acid;

(b) forcing the bolus to move through the microchannel;

(c) while the bolus is moving through the microchannel, (i) controlling a thermal generating apparatus to cycle the temperature of the bolus to amplify nucleic add contained in the bolus, wherein the thermal generating apparatus sequentially controls temperature amplification and thermal denaturation, the thermal denaturation being performed after the amplification is completed and (ii) determining at least once per temperature cycle whether the bolus has been exposed to at least a predetermined number of temperature cycles; and

(d) in response to determining that the bolus has been exposed to at least a predetermined number of temperature cycles, then, ceasing the temperature cycling and, while the bolus is still moving through the microchannel, controlling said thermal generating apparatus to cause thermal denaturation of dsDNA within the bolus to transition to ssDNA and using an image sensor to capture images of the bolus.

20. The method of claim 19, wherein the step of determining whether the bolus has been exposed to at least a predetermined number of temperature cycles comprises using the image sensor to capture an image of the bolus.

21. The method of claim 20, wherein the step of determining whether the bolus has been exposed to at least a predetermined number of temperature cycles further comprises processing the captured image data to determine the intensity of light emitted from the bolus.

22. The method of claim 21, wherein the step of determining whether the bolus has been exposed to at least a predetermined number of temperature cycles further comprises (a) comparing a value representing the determined intensity to a threshold value or (b) determining the rate of change in the intensity of the emissions from the bolus.

23. The method of claim 22, further comprising initiating a process for causing the dsDNA within the bolus to transition to ssDNA in response to determining that the result of the comparison indicates that the value representing the determined intensity is greater than the threshold value.

24. The method of claim 23, wherein the step of initiating the process comprises configuring the thermal generating apparatus to gradually increase the temperature of the bolus.

25. The method of claim 20, wherein the step of determining whether the bolus has been exposed to at least a predetermined number of temperature cycles further comprises using the image sensor to capture an image of the bolus at least once during each temperature cycle.