Collagen scaffolds, medical implants with same and methods of use

• US 9,579,422 B2
• Filed: 03/09/2015
• Issued: 02/28/2017
• Est. Priority Date: 06/22/2006
• Status: Active Grant

• Current Assignee: University of South Florida (State University System of Florida)
• Original Assignee: University of South Florida (State University System of Florida)
• Inventors: Ju, Young Min, Moussy, Francis, Koob, Thomas J.
• Primary Examiner(s): Carlson, Karen Cochrane
• Application Number:
  US14/642,137
• Publication Number:
  US 20150174296A1
• Time in Patent Office: 722 Days
• US Class Current: 1/1
• CPC Class Codes: A61B 5/14542 for measuring blood gases A61B5/14551 takes precedence
• CPC Class Codes: A61B 5/1486 using enzyme electrodes, e.g. with immobilised oxidase
• CPC Class Codes: A61B 5/14865 invasive, e.g. introduced into the body by a catheter or needle or using implanted sensors
• CPC Class Codes: A61L 2400/02 Treatment of implants to prevent calcification or mineralisation in vivo
• CPC Class Codes: A61L 2400/18 Modification of implant surfaces in order to improve biocompatibility, cell growth, fixation of biomolecules, e.g. plasma treatment
• CPC Class Codes: A61L 2420/02 Methods for coating medical devices
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: A61L 27/24 Collagen
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/48 with macromolecular fillers
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 27/56 Porous materials, e.g. foams or sponges
• CPC Class Codes: A61L 31/044 Collagen
• CPC Class Codes: A61L 31/10 Macromolecular materials
• CPC Class Codes: A61L 31/129 containing macromolecular fillers
• CPC Class Codes: C08L 89/06 derived from leather or skin , e.g. gelatin

Abstract

The subject invention concerns non-degradable three dimensional porous collagen scaffolds and coatings. These scaffolds can be prepared around sensors for implantation into a body. A specific embodiment of the invention concerns implantable glucose sensors. Sensors comprising a collagen scaffold of the invention have improved biocompatibility by minimizing tissue reactions while stimulating angiogenesis. The subject invention also concerns methods for preparing collagen scaffolds of the invention. The subject invention also concerns sensors that have a collagen scaffold of the invention around the exterior of the sensor.

Claims

1. A method for preparing a device for implantation into the body or tissue of a person or animal, said method comprising placing a biocompatible collagen scaffold or coating on said device, wherein said collagen scaffold or coating comprises regularly distributed open pores and an interconnected pore structure and is imbedded in a nordihydroquaiaretic acid (NDGA) bisquinone polymer matrix, and wherein said open pores are between about 10 um to about 200 um in diameter (mean).

2. The method according to claim 1, wherein said method comprises:

a) contacting said divice with a solution containing said biocompatible collagen scaffold or coating; and

b) drying said solution on said device.

3. The method according to claim 2, wherein steps (a) and (b) of said method are repeated at least one time.

4. The method according to claim 2, wherein steps (a) and (b) of said method are repeated at least two to four times.

5. The method according to claim 2, wherein said drying step comprises freeze drying.

6. The method according to claim 2, wherein said solution of step (a) comprises about 1% (w/v) collagen.

7. The method according to claim 1 wherein the mean pore size of the collagen scaffold is about 60 pm or less in diameter.

8. The method according to claim 1, wherein the mean pore size of the collagen scaffold is between 40 m and 80 m in diameter.

9. The method according to claim 1, wherein said collagen scaffold or coating comprises at least one of an antimicrobial, anti-inflammatory, and/or angiogenic compound, drug, or growth factor.

10. The method according to claim 1, further comprising integrating a hydrogel matrix into or on said collagen scaffold.

11. The method according to claim 1, wherein said collagen scaffold or coating comprises basic fibroblast growth factor, tumor growth factor beta, a bone morphogenic protein, platelet-derived growth factor, an insulin-like growth factor, fibronectin, hyaluronan, aggrecan, biglycan, or decorin.

12. The method according to claim 1, wherein said collagen is from fish, starfish, sea urchin, sponge, shark, skate, ray, equine, bovine, ovine, porcine, canine, or feline.

13. A method for providing an implantable device with a biocompatible collagen coating or scaffold, wherein sad collagen scaffold or coating comprises regularly distributed open pores and an interconnected pore structure and is imbedded in a nordihydroguaiaretic acid (NDGA) bisquinone polymer matrix, and wherein said open pores are between about 10 um to about 200 um in diameter (mean), said method comprising:

a) contacting an implantable device structure with a collagen containing solution; and

b) drying said collagen solution on said device.