×

DHAD variants and methods of screening

  • US 9,580,705 B2
  • Filed: 03/13/2014
  • Issued: 02/28/2017
  • Est. Priority Date: 03/15/2013
  • Status: Active Grant
First Claim
Patent Images

1. An isolated polypeptide having dihydroxy-acid dehydratase (DHAD) activity, wherein the polypeptide is a Streptococcus mutans DHAD and comprises one or more amino acid substitutions selected from:

  • (a) aspartic acid at a position corresponding to position 33 of SEQ ID NO;

    168;

    (b) glutamic acid at a position corresponding to position 62 of SEQ ID NO;

    168;

    (c) valine at a position corresponding to position 115 of SEQ ID NO;

    168;

    (d) glutamic acid at a position corresponding to position 116 of SEQ ID NO;

    168;

    (e) serine at a position corresponding to position 119 of SEQ ID NO;

    168;

    (f) arginine at a position corresponding to position 158 of SEQ ID NO;

    168;

    (g) glutamine at a position corresponding to position 176 of SEQ ID NO;

    168;

    (h) leucine at a position corresponding to position 179 of SEQ ID NO;

    168;

    (i) arginine at a position corresponding to position 322 of SEQ ID NO;

    168;

    (j) serine at a position corresponding to position 425 of SEQ ID NO;

    168;

    (k) glycine at a position corresponding to position 524 of SEQ ID NO;

    168;

    (l) valine or leucine at a position corresponding to position 562 of SEQ ID NO;

    168;

    (m) arginine, cysteine, or glycine at a position corresponding to position 563 of SEQ ID NO;

    168;

    (n) glutamic acid at a position corresponding to position 564 of SEQ ID NO;

    168; and

    (o) aspartic acid at a position corresponding to position 567 of SEQ ID NO;

    168.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×