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Protocols for making hepatocytes from embryonic stem cells

  • US 9,587,223 B2
  • Filed: 08/27/2013
  • Issued: 03/07/2017
  • Est. Priority Date: 04/27/2000
  • Status: Active Grant
First Claim
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1. A method for screening for a response of cultured hepatocyte lineage cells to an administered chemical compound, the method comprising:

  • a) obtaining a population of cultured cells that are in vitro differentiated progeny of cells expressing SSEA3, SSEA4, TRA-1-60 and TRA-1-81 and have morphological features of hepatocytes;

    b) administering the chemical compound to the population of cultured cells; and

    c) measuring a response of the population of cultured cells to the administered compound;

    wherein the response is selected from a change in the viability, survival, morphology, marker phenotype, metabolic activity, and/or leakage of enzymes into the culture medium of the cultured cells.

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