Substrates, systems and methods for analyzing materials

• US 9,587,276 B2
• Filed: 11/18/2015
• Issued: 03/07/2017
• Est. Priority Date: 09/01/2006
• Status: Active Grant

• Current Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Original Assignee: Pacific Bioscience of California Incorporated (L'Oreal SA)
• Inventors: Lundquist, Paul, Turner, Stephen
• Primary Examiner(s): Kim, Kiho
• Application Number:
  US14/945,256
• Publication Number:
  US 20160186255A1
• Time in Patent Office: 475 Days
• US Class Current: 1/1
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: C12Q 1/6869 Methods for sequencing
• CPC Class Codes: C12Q 1/6874 involving nucleic acid arrays, e.g. sequencing by hybridisation
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 2021/7786 Fluorescence
• CPC Class Codes: G01N 21/64 Fluorescence; Phosphorescence
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/645 Specially adapted constructive features of fluorimeters
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/648 using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
• CPC Class Codes: G01N 21/7703 using reagent-clad optical fibres or optical waveguides using measurement of total internal reflection or attenuated total reflection G01N21/552; optical fibres or waveguides per se G02B
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides
• CPC Class Codes: G02B 6/10 of the optical waveguide type G02B6/02, G02B6/24 take precedence; devices or arrangements for the control of light by electric, magnetic, electro-magnetic or acoustic means G02F1/00; transferring the modulation of modulated light G02F2/00; optical logic elements G02F3/00; optical analogue/digital converters G02F7/00
• CPC Class Codes: G02B 6/43 Arrangements comprising a plurality of opto-electronic elements and associated optical interconnections

Abstract

Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses.

Claims

1. A method of performing a sequencing by synthesis reaction, the method comprising:

a) providing a substrate comprising;

i) a first surface and a mask layer disposed over the first surface of the substrate, the mask layer comprising a plurality of apertures that pass through the mask layer, wherein the apertures are configured to spatially confine an individual, optically resolvable complex;

ii) at least a first optical waveguide disposed beneath but sufficiently proximal to the plurality of apertures that when excitation radiation is passed through the first optical waveguide, an evanescent field emanating from the first optical waveguide reaches into the apertures;

b) immobilizing an individual polymerase/template/primer complex within one of the apertures sufficiently proximal to the first optical waveguide to be illuminated by the evanescent field;

c) passing excitation light through the first optical waveguide, thereby generating the evanescent field;

d) exposing the polymerase/template/primer complex to a set of labeled nucleotides or nucleotide analogs under conditions that promote nascent strand synthesis; and

e) detecting emissions from said one of the apertures, wherein said emissions are emitted from the labeled nucleotides or nucleotide analogs during said nascent strand synthesis, and are indicative of a sequence being synthesized, thereby performing a sequencing by synthesis reaction.

2. The method of claim 1, wherein the labeled nucleotides or nucleotide analogs comprise fluorescent labels.

3. The method of claim 2, wherein the fluorescent labels on the labeled nucleotides or nucleotide analogs are removed during said nascent strand synthesis.

4. The method of claim 1, wherein the substrate further comprises at least one integrated optical functionality selected from the group consisting of mask layers, lenses, filters, gratings, antireflective coatings, optical switching components, or attenuation components.

5. The method of claim 1, wherein said passing excitation light through the first optical waveguide comprises optically coupling at least a first excitation radiation source to the first optical waveguide.

6. The method of claim 1, wherein said passing excitation light through the first optical waveguide comprises optically coupling at least two excitation radiation sources to the first optical waveguide.

7. The method of claim 6, wherein the at least two excitation radiation sources provide additional intensity to the excitation light.

8. The method of claim 1, wherein said mask layer comprises at least one material selected from the group consisting of a metal, a polymeric material, and a silica-based material.

9. The method of claim 1, wherein said mask layer is a transparent mask layer.

10. The method of claim 1, wherein the polymerase/template/primer complex comprises a tag sequence ligated to a template sequence and a primer annealed to the tag sequence.

11. A method for analyzing an analyte, comprising:

a) providing a substrate comprising;

i) a first surface;

ii) a mask layer disposed over the first surface, the mask layer comprising a plurality of apertures that pass through the mask layer; and

iii) at least a first optical waveguide disposed beneath but sufficiently proximal to at least a first aperture in the plurality of apertures that when excitation radiation is passed through the first optical waveguide, an evanescent field emanating from the first optical waveguide reaches into at least the first aperture;

b) disposing an optically resolvable individual analyte within said first aperture, wherein the analyte is disposed sufficiently proximal to the first optical waveguide to be illuminated by the evanescent field;

c) directing light into the first optical waveguide, thereby generating the evanescent field and, in doing so, illuminating the analyte; and

d) detecting an optical signal from the analyte disposed within said first aperture, thereby analyzing the analyte.

12. The method of claim 11, wherein said directing light into the first optical waveguide excites a fluorescent moiety linked to the optically resolvable individual analyte, and further wherein said detecting an optical signal from the analyte comprises detecting a fluorescent signal from the fluorescent moiety.

13. The method of claim 11, wherein the substrate comprises a plurality of optical waveguides disposed beneath but sufficiently proximal to the plurality of apertures such that when excitation radiation is passed through the plurality of optical waveguides, a plurality of evanescent fields emanating from the plurality of optical waveguide reaches into the plurality of apertures.

14. The method of claim 11, wherein said directing light into the first optical waveguide comprises directing light from multiple light sources into the first optical waveguide.

15. The method of claim 11, wherein the first optical waveguide is optically coupled to two or more originating waveguides disposed beneath the first surface.

16. The method of claim 11, wherein the substrate further comprises at least one integrated optical functionality selected from the group consisting of mask layers, lenses, filters, gratings, antireflective coatings, optical switching components, or attenuation components.

17. The method of claim 11, wherein the optically resolvable individual analyte is a binding partner for an immobilized binding agent in said one of the apertures.

18. The method of claim 11, wherein said disposing the optically resolvable individual analyte comprises interaction of the optically resolvable individual analyte with an immobilized enzyme complex.

19. The method of claim 18, wherein the optically resolvable individual analyte is a fluorescently labeled nucleotide and the immobilized enzyme complex is a polymerase/template/primer complex.

20. The method of claim 19, wherein said first aperture contains only one polymerase/template/primer complex.