Flow control in microfluidic systems

• US 9,592,505 B2
• Filed: 06/06/2012
• Issued: 03/14/2017
• Est. Priority Date: 04/25/2008
• Status: Active Grant

• Current Assignee: Opko Diagnostics, LLC (Opko Health, Inc.)
• Original Assignee: Opko Diagnostics, LLC (Opko Health, Inc.)
• Inventors: Linder, Vincent, Steinmiller, David
• Primary Examiner(s): MARTINEZ, REBECCA L
• Application Number:
  US13/490,033
• Publication Number:
  US 20120241013A1
• Time in Patent Office: 1,742 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/0673 Handling of plugs of fluid surrounded by immiscible fluid
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 2400/082 Active control of flow resistance, e.g. flow controllers
• CPC Class Codes: B01L 2400/084 Passive control of flow resistance
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: B01L 3/502784 specially adapted for droplet or plug flow, e.g. digital microfluidics automatic analysis using a stream of discrete samples in a tube system G01N35/08
• CPC Class Codes: Y10T 137/0318 Processes
• CPC Class Codes: Y10T 137/0324 With control of flow by a condition or characteristic of a fluid

Abstract

Microfluidic systems and methods including those that provide control of fluid flow are provided. Such systems and methods can be used, for example, to control pressure-driven flow based on the influence of channel geometry and the viscosity of one or more fluids inside the system.

Claims

1. A device comprising:

a microfluidic system, wherein the microfluidic system comprises;

a first plug comprising a first fluid and a second plug comprising a second fluid contained in the microfluidic system, wherein the first and second plugs are separated by a gas, and wherein the first plug, the second plug, and the gas are present in the microfluidic system prior to first use of the device;

an inlet and an outlet;

a reaction area positioned between the inlet and the outlet;

a microfluidic channel in fluid communication with and downstream of the reaction area, the microfluidic channel comprising a first channel portion having a first cross-sectional area; and

a flow constriction region positioned downstream of the first channel portion and upstream of the outlet, wherein the flow constriction region has a second cross-sectional area, the second cross-sectional area being less than 250 μ

m² and at least 10 times smaller than the first cross-sectional area, wherein the flow constriction region has a length of at least 1 cm, and wherein the flow constriction region is adapted and arranged to substantially stop flow of a liquid in the microfluidic system.

2. A device as in claim 1, wherein the second cross-sectional area is less than 50 μ

m².

3. A device as in claim 1, wherein the second cross-sectional area is less than 100 μ

m².

4. A device as in claim 1, wherein the second cross-sectional area is at least 25 times smaller than the first cross-sectional area.

5. A device as in claim 1, wherein the flow constriction region has a length of at least 2 cm.

6. A device as in claim 1, wherein the flow constriction region comprises a serpentine configuration.

7. A device as in claim 1, wherein the first channel portion comprises a serpentine configuration.

8. A device as in claim 7, comprising a detector associated with the first channel portion.

9. A device as in claim 1, comprising a liquid containment region positioned upstream of the flow constriction region, wherein the liquid containment region comprises an absorbent material.

10. A device as in claim 1, wherein the microfluidic system does not include any moving parts.

11. A device as in claim 1, wherein the flow constriction region is positioned adjacent the outlet of the microfluidic system.

12. A device as in claim 1, comprising a source of vacuum connected to the outlet of the microfluidic system.

13. A device as in claim 12, comprising a liquid in the microfluidic system that is substantially prevented from exiting the outlet of the microfluidic system when the source of vacuum is applied.

14. A device as in claim 1, wherein the first and second plugs of fluid are stored and sealed in the device.

15. A device as in claim 1, wherein the first and second plugs are stored and sealed in a microfluidic channel of the device.

16. A device as in claim 1, wherein the first and/or second fluid is a buffer solution.

17. A device as in claim 1, wherein the first and/or second fluid comprises a reagent for a chemical and/or biological reagent.

18. A device as in claim 1, wherein a viscosity of the first fluid and a viscosity of the second fluid differs by a factor of at least 3.

19. A device as in claim 1, wherein the flow constriction region is adapted and arranged to substantially stop flow of the second plug by flow of the first plug into the flow constriction region.

20. A device as in claim 1, wherein the flow constriction region is a first flow constriction region, the device comprising a second flow constriction region, wherein the second flow constriction region is positioned upstream of the reaction area, and wherein the second flow constriction region has a third cross-sectional area, the third cross-sectional area being at least 10 times smaller than the first cross-sectional area.