Differentiation of pluripotent stem cells
First Claim
1. A method to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage, comprising treating the pluripotent stem cells with a medium lacking activin A, and containing GDF-8 and a cyclic analine-pyridinotriazine comprising 5-Chloro-1,8,10,12,16,22,26,32-octaazapentacyclo[24.2.2.1˜
- 3,7˜
.1˜
9,13˜
.1˜
14,18˜
]tritriaconta-3(33),4,6,9(32),10,12,14(31),15,17-nonaen-23-one, for a period of time sufficient for the pluripotent stem cells to differentiate into cells expressing markers characteristic of the definitive endoderm lineage.
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Accused Products
Abstract
The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention is directed to methods and compositions to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage comprising culturing the pluripotent stem cells in medium comprising a sufficient amount of GDF-8 to cause the differentiation of the pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage.
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Citations
13 Claims
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1. A method to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage, comprising treating the pluripotent stem cells with a medium lacking activin A, and containing GDF-8 and a cyclic analine-pyridinotriazine comprising 5-Chloro-1,8,10,12,16,22,26,32-octaazapentacyclo[24.2.2.1˜
- 3,7˜
.1˜
9,13˜
.1˜
14,18˜
]tritriaconta-3(33),4,6,9(32),10,12,14(31),15,17-nonaen-23-one, for a period of time sufficient for the pluripotent stem cells to differentiate into cells expressing markers characteristic of the definitive endoderm lineage. - View Dependent Claims (2, 3, 4, 5, 12, 13)
- 3,7˜
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6. A method of enhancing the differentiation of pluripotent stem cells into definitive endoderm lineage cells comprising differentiating pluripotent stem cells into definitive endoderm cells by culturing the pluripotent stem cells for about one to about three days with a medium lacking activin A, and containing GDF-8 and 5-Chloro-1,8,10,12,16,22,26,32-octaazapentacyclo[24.2.2.1˜
- 3,7˜
.1˜
9,13˜
.1˜
14,18˜
]tritriaconta-3(33),4,6,9(32),10,12,14(31),15,17-nonaen-23-one. - View Dependent Claims (7, 8)
- 3,7˜
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9. A method of enhancing the yield of definitive endoderm cells in a population comprising definitive endoderm cells comprising:
- differentiating a population of pluripotent stem cells into the population comprising definitive endoderm cells by culturing the pluripotent stem cells in a medium lacking activin A, and containing GDF-8 and 5-Chloro-1,8,10,12,16,22,26,32-octaazapentacyclo[24.2.2.1˜
3,7˜
.1˜
9,13˜
.1˜
14,18˜
]tritriaconta-3(33),4,6,9(32),10,12,14(31),15,17-nonaen-23-one. - View Dependent Claims (10, 11)
- differentiating a population of pluripotent stem cells into the population comprising definitive endoderm cells by culturing the pluripotent stem cells in a medium lacking activin A, and containing GDF-8 and 5-Chloro-1,8,10,12,16,22,26,32-octaazapentacyclo[24.2.2.1˜
Specification