Molecular recognition matrix and method for making same

• US 9,594,080 B2
• Filed: 01/26/2010
• Issued: 03/14/2017
• Est. Priority Date: 03/09/2009
• Status: Active Grant

• Current Assignee: Yanxiu Zhou
• Original Assignee: Yanxiu Zhou
• Inventors: Zhou, Yanxiu
• Primary Examiner(s): BROWN, MELANIE YU
• Application Number:
  US12/694,088
• Publication Number:
  US 20100226984A1
• Time in Patent Office: 2,604 Days
• US Class Current: 1/1
• CPC Class Codes: G01N 33/54386 Analytical elements

Abstract

A molecular recognition matrix which utilizes the interaction between molecular building blocks and the surface of a substrate to develop specific molecular recognition cavities.

Claims

1. A method for making a molecular recognition matrix comprising, in order, the steps of:

providing one or more molecules for use as molecule building blocks and a substrate having a surface capable of interacting with the molecule building blocks;

providing one or more template molecules;

adding the molecule building blocks and the template molecules separately to one or more solvents to create a deposition solution wherein the molecule building blocks are soluble in the one or more solvents;

adding the substrate to the deposition solution to create a matrix via the interaction between the molecule building blocks and the surface of the substrate where the molecule building blocks interact with the surface of the substrate and there is no bonding between the molecule building blocks and the templates;

removing the substrate containing the matrix from the deposition solution; and

rinsing the substrate containing the matrix with a different one or more solvents or other solutions to extract the template molecules thereby creating an imprinted substrate.

2. The method of claim 1 further comprising the step of curing the imprinted substrate.

3. The method of claim 1 wherein the molecule building blocks comprise at least one of a monomer, a molecule, an ion, a polyatomic ion, a salt, a compound, a complex, a pure chemical element, an elemental molecule, and a crystal.

4. The method of claim 1 wherein the substrate comprises a solid material comprising at least one of a polymer, an optical fiber, a metal, a semiconductor, a glass, a plastic, an organic material, and an inorganic material.

5. The method of claim 1 wherein the substrate may comprise any shape.

6. The method of claim 1 wherein the substrate may comprise any size of material from bulk size materials to nanometer size materials.

7. The method of claim 1 wherein the template molecules comprise at least one or more kinds of molecules including one or more of a small molecule, a biological macromolecule, a microorganism, a crystal, a virus, or any material needing detection, separation, extraction, identification, capturing, or any other functional process.

8. The method of claim 1 wherein at least one molecular recognition cavity is formed on the surface of the substrate.

9. The method of claim 1 wherein the interaction between the substrate surface and the molecular building blocks comprises at least one of covalent bonding, non-covalent bonding, hydrophobic forces, van der walls forces, pi-pi interactions, ionic interactions, electrostatic effects, and other interactions that keep the template molecules within the matrix formed within the deposition solution.

10. The method of claim 1 wherein the interaction between the molecule building blocks and the surface of the substrate may be via polymerization, a self-assembling process, a polymerization after self-assembling process, an electro-polymerization, or other reaction process.

11. The method of claim 1 wherein the molecule building blocks are synthesized to have a specific function to interact with the surface of the substrate.

12. The method of claim 1 further comprising the step of pre-modifying or pre-treating the surface of the substrate with one or more specific functional groups that interact with the molecule building blocks.

13. The method of claim 1 further comprising the step of carrying out a post-synthetic functionalization process to increase at least one of the affinity and selectivity of the imprinted substrate after exposing the imprinted substrate.

14. A method for making a molecular recognition matrix comprising, in order, the steps of:

providing one or more molecules for use as molecule building blocks and a substrate having a surface capable of interacting with the molecule building blocks;

providing one or more template molecules;

adding the molecule building blocks and the template molecules to one or more solvents to create a deposition solution wherein the molecule building blocks are soluble in the one or more solvents;

adding the substrate to the deposition solution to create a matrix via the interaction between the molecule building blocks and the surface of the substrate where the molecule building blocks interact with the surface of the substrate and there is no bonding between the molecule building blocks and the templates;

removing the substrate containing the matrix from the deposition solution; and

rinsing the substrate containing the matrix with a different one or more solvents or other solutions to extract the template molecules thereby creating an imprinted substrate.