T cell modifying compounds and uses thereof
First Claim
1. A genetically modified T-cell that expresses a chimeric antigen receptor (CAR), wherein an exogenous sequence encoding a CAR is integrated into the genome of the T-cell using one or more zinc finger nucleases, and further wherein expression of at least one endogenous immunological checkpoint gene is repressed by genetically modifying the immunological checkpoint gene in the T-cell, wherein the immunological checkpoint gene is a programmed death receptor PDCD1 gene or a CTLA-4 gene, wherein the genetic modification is to a sequence as shown in any SEQ ID NO:
- 21-33 and 116-120.
2 Assignments
0 Petitions
Accused Products
Abstract
Methods and compositions for modifying T-cells in which PD1 and/or CTLA-4 is repressed and/or inactivated using fusion proteins such as artificial transcription factors and nucleases.
146 Citations
23 Claims
- 1. A genetically modified T-cell that expresses a chimeric antigen receptor (CAR), wherein an exogenous sequence encoding a CAR is integrated into the genome of the T-cell using one or more zinc finger nucleases, and further wherein expression of at least one endogenous immunological checkpoint gene is repressed by genetically modifying the immunological checkpoint gene in the T-cell, wherein the immunological checkpoint gene is a programmed death receptor PDCD1 gene or a CTLA-4 gene, wherein the genetic modification is to a sequence as shown in any SEQ ID NO:
Specification