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Methods for culturing and analyzing cells

  • US 9,597,644 B2
  • Filed: 03/26/2010
  • Issued: 03/21/2017
  • Est. Priority Date: 09/05/2003
  • Status: Active Grant
First Claim
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1. A method for analyzing a biological sample using an analysis system, the method comprising the steps of:

  • acquiring the biological sample from a sample source using a sample acquisition device;

    transferring the acquired biological sample to a droplet generator, the droplet generator comprising a liquid bridge,providing a flow of cell culture media to the droplet generator,mixing the biological sample and cell culture media at the liquid bridge,segmenting, using the droplet generator, the mixed acquired biological sample and cell culture media into a plurality of individually mixed wrapped droplets, wherein the droplets are wrapped in an immiscible carrier fluid using the droplet generator and comprise at least the biological sample, the cell culture media, and a reagent;

    collecting the wrapped sample droplet in a vessel, the vessel configured to contain a carrier fluid for receiving and protecting the wrapped sample droplet;

    flowing the wrapped droplet through the analysis system; and

    analyzing the wrapped droplet to detect products of a polymerase chain reaction.

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