×

Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose

  • US 9,598,682 B2
  • Filed: 09/29/2010
  • Issued: 03/21/2017
  • Est. Priority Date: 09/29/2009
  • Status: Active Grant
First Claim
Patent Images

1. A method for uncapping a mannose-6-phosphate residue on an oligosaccharide, said method comprisinga) providing said oligosaccharide having a mannose-1-phospho-6-mannose linkage;

  • andb) contacting said oligosaccharide with a mannosidase capable of hydrolyzing said mannose-1-phospho-6-mannose residue to phospho-6-mannose, wherein said mannosidase is a member of glycosyl hydrolase family 92, wherein said mannosidase comprises an amino acid sequence having at least 95% identity to residues 1 to 774 of SEQ ID NO;

    50 or at least 98% identity to SEQ ID NO;

    50.

View all claims
  • 5 Assignments
Timeline View
Assignment View
    ×
    ×