Hydrolysis of mannose-1-phospho-6-mannose linkage to phospho-6-mannose
First Claim
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1. A method for uncapping a mannose-6-phosphate residue on an oligosaccharide, said method comprisinga) providing said oligosaccharide having a mannose-1-phospho-6-mannose linkage;
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50 or at least 98% identity to SEQ ID NO;
50.
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Abstract
Described herein are methods and genetically engineered cells useful for uncapping a mannose-6-phosphate residue on an oligosaccharide.
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Citations
21 Claims
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1. A method for uncapping a mannose-6-phosphate residue on an oligosaccharide, said method comprising
a) providing said oligosaccharide having a mannose-1-phospho-6-mannose linkage; - and
b) contacting said oligosaccharide with a mannosidase capable of hydrolyzing said mannose-1-phospho-6-mannose residue to phospho-6-mannose, wherein said mannosidase is a member of glycosyl hydrolase family 92, wherein said mannosidase comprises an amino acid sequence having at least 95% identity to residues 1 to 774 of SEQ ID NO;
50 or at least 98% identity to SEQ ID NO;
50. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification