Sample preparation paper cartridge

• US 9,604,216 B2
• Filed: 02/11/2014
• Issued: 03/28/2017
• Est. Priority Date: 02/11/2013
• Status: Active Grant

• Current Assignee: Epistem Limited
• Original Assignee: Epistem Limited
• Inventors: Cobb, Benjamin
• Primary Examiner(s): Levkovich, Natalia
• Application Number:
  US14/767,265
• Publication Number:
  US 20160030939A1
• Time in Patent Office: 1,141 Days
• Field of Search: 422/554, 422/430
• US Class Current: 1/1
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 2300/022 Transponder chips
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0681 Filter
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 2300/126 Paper
• CPC Class Codes: B01L 2300/16 Surface properties and coatings
• CPC Class Codes: B01L 2300/165 Specific details about hydrophobic, oleophobic surfaces
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0633 with moving parts
• CPC Class Codes: B01L 3/5023 with a sample being transported to, and subsequently stored in an absorbent for analysis
• CPC Class Codes: B01L 3/52 Containers specially adapted for storing or dispensing a reagent B01L3/02 takes precedence; containers for medical or pharmaceutical purposes A61J1/00; containers in general B65D; storing or dispensing test elements G01N33/4875; automated reagent dispensing G01N35/1002
• CPC Class Codes: C12N 15/1003 Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
• CPC Class Codes: G01N 1/28 Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Qmounting specimens on microscopic slides G02B21/34; means for supporting the objects or the materials to be analysed in electron microscopes H01J37/20 ; laboratory gas handling apparatus B01L5/00

Abstract

A sample preparation cartridge is described having a lower portion having four integrally-formed leaf springs, and a raised lower base part which is slightly below the level of the leaf springs. An upper portion of the cartridge engages with the lower portion, via four paired pins and holes formed in the lower and upper portions. The upper portion includes three well-shaped openings formed in the central region of the upper portion. Between the upper and lower portions is placed a sheet of sample preparation paper, resting on the leaf springs, which raise the lower face of the paper away from the raised lower base part, leaving a small air gap between the base and the paper. The upper face of the paper is in contact with the well-shaped openings of the upper portion. The user loads a liquid sample into the openings, and the paper wicks the liquid sample away from the loading location, leaving cellular debris in the place of application. Applying pressure to the upper portion of the cartridge urges the paper into contact with the raised base portion, so providing a firm base which allows the user to remove sections of the paper, using for example a biopsy needle, for further processing.

Claims

1. A sample preparation cartridge comprising:

a lower portion having a base and a raised portion projecting from the base;

at least one sprung member projecting from the base of the lower portion;

an upper portion engageable with and removable from the lower portion, and comprising at least one well shaped opening;

wherein

the at least one sprung member is located between the lower and upper portions when the upper portion is engaged with the lower portion;

the at least one sprung member projects from the lower portion further than the raised portion;

and wherein the raised portion is configured to align with the well shaped opening,

the cartridge being configured such that a sample preparation paper placed between the upper and lower portions located adjacent the well shaped opening rests on the at least one sprung member so as to separate the paper from the lower portion; and

wherein when a user applies pressure to the upper portion the at least one sprung member compresses, and thereby urges the paper into contact with the raised portion of the lower portion.

2. The cartridge of claim 1 comprising two sprung members.

3. The cartridge of claim 1 wherein the at least one sprung member is formed integrally with the lower portion.

4. The cartridge of claim 1 wherein the upper portion is made from a plastics material.

5. The cartridge of claim 1 wherein the upper portion is translucent and/or clear.

6. The cartridge of claim 4 wherein the plastics material is hydrophobic.

7. The cartridge of claim 1 further comprising a sample preparation paper located between the upper and lower portions.

8. The cartridge of claim 7 wherein the sample preparation paper is a composite paper.

9. The cartridge of claim 7 or 8 wherein the sample preparation paper is functionalised with one or more biocidal agents capable of i) weakening cell membrane, cell wall, viral envelope, or viral capsid of biological material in a sample;

or ii) lysing cellular or viral material in a sample.

10. The cartridge of claim 9 wherein the biocidal agent comprises multiple functional groups, including a binding moiety;

a hydrophobic moiety; and

a charged moiety.

11. The cartridge of claim 10 wherein the hydrophobic moiety is an alkyl chain.

12. The cartridge of claim 10 wherein the charged moiety is positively charged.

13. The cartridge of claim 10 wherein the binding moiety comprises a hydroxyl group.

14. The cartridge of claim 9 wherein the biocidal agent is a silylated quaternary ammonium compound (SiQAC).

17. The cartridge of claim 1 comprising four sprung members.

18. The cartridge of claim 4 wherein the plastics material is modified with groups to assist in lysis of cellular material in samples.

19. The cartridge of claim 18 wherein the plastics material is functionalised with a silylated quaternary ammonium compound (SiQAC).

20. The cartridge of claim 19 wherein the plastics material is functionalised with 3-(trimethoxysilyl) propyldimethyloctadecyl ammonium chloride (3-TPAC).

21. The cartridge of claim 8 wherein the paper is a composite cellulose paper comprising a lateral flow layer.

22. The cartridge of claim 11 wherein the hydrophobic moiety is C5-C30 alkyl.

23. The cartridge of claim 11 wherein the hydrophobic moiety is C10-C20 alkyl.

24. The cartridge of claim 12 wherein the charged moiety is a quaternary ammonium group.

25. The cartridge of claim 14 wherein the biocidal agent is 3-(trimethoxysilyl) propyldimethyloctadecyl ammonium chloride (3-TPAC).

15. A kit comprising:

a) a sample preparation cartridge comprising;

a lower portion having a base and a raised portion projecting from the base;

at least one sprung member projecting from the base of the lower portion;

an upper portion engageable with and removable from the lower portion, and comprising at least one well shaped opening;

wherein

the at least one sprung member is located between the lower and upper portions when the upper portion is engaged with the lower portion;

the at least one sprung member projects from the base of the lower portion further than the raised portion,

and wherein the raised portion is configured to align with the well shaped opening;

the cartridge being configured such that a sample preparation paper placed between the upper and lower portions located adjacent the well shaped opening rests on the at least one sprung member so as to separate the paper from the base of the lower portion;

wherein when a user applies pressure to the upper portion the at least one sprung member compresses, and thereby urges the paper into contact the raised portion of the lower portion; and

b) a sample preparation paper.

16. The kit of claim 15, wherein the sample preparation paper is a composite paper.