Protein scaffolds for antibody mimics and other binding proteins

• US 9,605,039 B2
• Filed: 10/03/2006
• Issued: 03/28/2017
• Est. Priority Date: 12/10/1998
• Status: Expired due to Term

• Current Assignee: Bristol-Myers Squibb Company
• Original Assignee: Bristol-Myers Squibb Company
• Inventors: Lipovsek, Dasa, Wagner, Richard W., Kuimelis, Robert G.
• Primary Examiner(s): AUDET, MAURY A
• Application Number:
  US11/543,316
• Publication Number:
  US 20070082365A1
• Time in Patent Office: 3,829 Days
• Field of Search: None
• US Class Current: 1/1
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 14/525 Tumour necrosis factor [TNF]
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 16/241 Tumor Necrosis Factors
• CPC Class Codes: C07K 2317/22 from camelids, e.g. camel, llama or dromedary
• CPC Class Codes: C07K 2318/20 Antigen-binding scaffold molecules wherein the scaffold is not an immunoglobulin variable region or antibody mimetics
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 2319/30 Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
• CPC Class Codes: C12N 15/1037 Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
• CPC Class Codes: C12Q 1/00 Measuring or testing processes involving enzymes, nucleic acids or microorganisms measuring or testing apparatus with condition measuring or sensing means, e.g. colony counters, C12M1/34; Compositions therefor; Processes of preparing such compositions
• CPC Class Codes: C40B 40/02 Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors

Abstract

Disclosed herein are proteins that include an immunoglobulin fold and that can be used as scaffolds. Also disclosed herein are nucleic acids encoding such proteins and the use of such proteins in diagnostic methods and in methods for evolving novel compound-binding species and their ligands.

Claims

1. An isolated protein comprising a domain derived from the tenth module of the human fibronectin type III (¹⁰Fn3), wherein said domain comprises

the amino acid sequence selected from the group consisting of:

SEQ ID NOs;

80, 96, 67, 127, 128, 135, and 140.

2. The protein of claim 1, wherein said protein is a monomer under physiological conditions.

3. The protein of claim 1, wherein said protein is fused to a second protein domain.

4. The protein of claim 3, wherein said second protein domain is the constant region of an IgG (Fc).

5. The protein of claim 4, wherein said protein is a dimer under physiological conditions.

6. A pharmaceutical composition comprising the protein of claim 1.